Preparation of Articular Cartilage Specimens for Scanning Electron Microscopy
- Authors: Stupina T.A.1
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Affiliations:
- Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics, the Ministry of Health of the Russian Federation
- Issue: Vol 161, No 4 (2016)
- Pages: 558-560
- Section: Methods
- URL: https://journal-vniispk.ru/0007-4888/article/view/237593
- DOI: https://doi.org/10.1007/s10517-016-3460-9
- ID: 237593
Cite item
Abstract
We developed and adapted a technology for preparation of articular cartilage specimens for scanning electron microscopy. The method includes prefixation processing, fixation, washing, and dehydration of articular cartilage specimens with subsequent treatment in camphene and air-drying. The technological result consists in prevention of deformation of the articular cartilage structures. The method is simpler and cheaper than the known technologies.
About the authors
T. A. Stupina
Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics, the Ministry of Health of the Russian Federation
Author for correspondence.
Email: stupinasta@mail.ru
Russian Federation, Kurgan
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