Enviar artigo por via de e-mail Preparation of Recombinant Serpin from Red King Crab Paralithodes сamtschaticus for Biomedical Research Purposes
- Autores: Kostin N.N.1,2, Ponomarenko N.A.1, Isaev V.A.3, Rudenskaya G.N.1, Bobik T.V.1, Gabibov A.G.1,2, Smirnov I.V.1,2
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Afiliações:
- M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
- Faculty of Chemistry, M. V. Lomonosov Moscow State University
- TRINITA Research and Production Company
- Edição: Volume 163, Nº 2 (2017)
- Páginas: 210-213
- Seção: Article
- URL: https://journal-vniispk.ru/0007-4888/article/view/238758
- DOI: https://doi.org/10.1007/s10517-017-3768-0
- ID: 238758
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Somente assinantes
Resumo
Genetic constructs with different leader sequences for intra- and extracellular expression of the target protein were generated and an original method for effective selection of clones with maximum expression was developed. For intracellular expression in the Pichia pastoris system, seprin content in cells was 6 mg/liter.
Sobre autores
N. Kostin
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences; Faculty of Chemistry, M. V. Lomonosov Moscow State University
Email: ivansmr@inbox.ru
Rússia, Moscow; Moscow
N. Ponomarenko
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
Email: ivansmr@inbox.ru
Rússia, Moscow
V. Isaev
TRINITA Research and Production Company
Email: ivansmr@inbox.ru
Rússia, Moscow Region
G. Rudenskaya
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
Email: ivansmr@inbox.ru
Rússia, Moscow
T. Bobik
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences
Email: ivansmr@inbox.ru
Rússia, Moscow
A. Gabibov
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences; Faculty of Chemistry, M. V. Lomonosov Moscow State University
Email: ivansmr@inbox.ru
Rússia, Moscow; Moscow
I. Smirnov
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Medical Sciences; Faculty of Chemistry, M. V. Lomonosov Moscow State University
Autor responsável pela correspondência
Email: ivansmr@inbox.ru
Rússia, Moscow; Moscow
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