Isolation of primary osteoblast cell lines from adult rat and rat embryos and their use as models for in vitro biocompatibility tests of nanostructured titanium-based implants


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Methods for obtaining osteoblast cultures from the calvaria of adult Wistar rats and 12-day-old embryos of these rats have been adapted for studying the biocompatibility and ossointegration of titanium-based implants. The osteoblast morphology and their differentiation into osteocytes on a titanium matrix with specially treated surface have been studied. It has been confirmed that two cultures of diploid rat cells obtained in the study can serve as efficient models for preclinical in vitro testing of nanostructured titanium implants for biocompatibility and osseointegration.

作者简介

I. Vyalykh

Ural Scientific Research Veterinary Institute

Email: juliakhrunyk@yahoo.co.uk
俄罗斯联邦, Yekaterinburg, 620000

A. Korelin

Ural Federal University

Email: juliakhrunyk@yahoo.co.uk
俄罗斯联邦, Yekaterinburg, 620002

S. Belikov

Ural Federal University

Email: juliakhrunyk@yahoo.co.uk
俄罗斯联邦, Yekaterinburg, 620002

M. Karabanalov

Ural Federal University

Email: juliakhrunyk@yahoo.co.uk
俄罗斯联邦, Yekaterinburg, 620002

S. Rakitin

Institute of Plant and Animal Ecology, Ural Branch

Email: juliakhrunyk@yahoo.co.uk
俄罗斯联邦, Yekaterinburg, 620144

R. Kamalov

Ural Federal University

Email: juliakhrunyk@yahoo.co.uk
俄罗斯联邦, Yekaterinburg, 620002

A. Popov

Ural Federal University

Email: juliakhrunyk@yahoo.co.uk
俄罗斯联邦, Yekaterinburg, 620002

Y. Khrunyk

Ural Federal University; Institute of Plant and Animal Ecology, Ural Branch

编辑信件的主要联系方式.
Email: juliakhrunyk@yahoo.co.uk
俄罗斯联邦, Yekaterinburg, 620002; Yekaterinburg, 620144

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