Preparation and Crystallization of Picornain 3C of Rhinovirus A28

A. E. Tishin; Тишин А. Е.; A. V. Gladysheva; Гладышева А. В.; L. A. Pyatavina; Пятавина Л. А.; S. E. Olkin; Олькин С. Е.; A. A. Gladysheva; Гладышева А. А.; I. R. Imatdionov; Иматдинов И. Р.; A. V. Vlaskina; Власкина А. В.; A. Yu. Nikolaeva; Николаева А. Ю.; V. R. Samygina; Самыгина В. Р.; A. P. Agafonov; Агафонов А. П.

doi:10.31857/S0023476123600313

Preparation and Crystallization of Picornain 3C of Rhinovirus A28

作者: Tishin A.E.¹, Gladysheva A.V.², Pyatavina L.A.¹^,3, Olkin S.E.¹, Gladysheva A.A.²^,4, Imatdionov I.R.¹, Vlaskina A.V.⁵, Nikolaeva A.Y.⁵, Samygina V.R.⁶^,7, Agafonov A.P.¹
隶属关系:
1. State Research Center of Virology and Biotechnology “Vector,” Rospotrebnadzor, 630559, Koltsovo, Novosibirsk oblast, Russia
2. State Scientific Center of Virology and Biotechnology «Vector»
3. Novosibirsk National Research State University, 630090, Novosibirsk, Russia
4. Novosibirsk National Research State University
5. National Research Centre “Kurchatov Institute”, 123098, Moscow, Russia
6. National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia
7. Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia
期: 卷 68, 编号 6 (2023)
页面: 926-933
栏目: STRUCTURE OF MACROMOLECULAR COMPOUNDS
URL: https://journal-vniispk.ru/0023-4761/article/view/231837
DOI: https://doi.org/10.31857/S0023476123600313
EDN: https://elibrary.ru/HZHBKY
ID: 231837

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详细

Human rhinovirus picornain 3C is a high-value commercial cysteine protease, which is widely used to remove affinity tags and fusion proteins during the purification of the target proteins. A variant of rhinovirus A28 picornain 3C produced in this study is not annotated in the NCBI databases, shares 79% sequence identity in the PDB, and was not previously used in the protein engineering. A protocol was developed for the isolation and purification of the protein to use it in structural studies. The initial crystallization conditions were found. The determination and analysis of the structure of rhinovirus A28 picornain 3C will provide new possibilities for performing basic research on the evolution of proteolytic enzymes and for the design of the optimal variant of this protease.

作者简介

A. Tishin

State Research Center of Virology and Biotechnology “Vector,” Rospotrebnadzor, 630559, Koltsovo, Novosibirsk oblast, Russia

Email: gladysheva_av@vector.nsc.ru
Россия, Кольцово

A. Gladysheva

State Scientific Center of Virology and Biotechnology «Vector»

Email: gladysheva_av@vector.nsc.ru
ORCID iD: 0000-0002-7396-3954
SPIN 代码: 5214-3421
Scopus 作者 ID: 57194590629

Postgraduate student, Juniour Researcher, Department of Molecular Virology for Flaviviruses and Viral Hepatitis

俄罗斯联邦, 630559, Novosibirsk region, Koltsovo

L. Pyatavina

State Research Center of Virology and Biotechnology “Vector,” Rospotrebnadzor, 630559, Koltsovo, Novosibirsk oblast, Russia; Novosibirsk National Research State University, 630090, Novosibirsk, Russia

Email: gladysheva_av@vector.nsc.ru
Россия, Кольцово; Россия, Новосибирск

S. Olkin

State Research Center of Virology and Biotechnology “Vector,” Rospotrebnadzor, 630559, Koltsovo, Novosibirsk oblast, Russia

Email: gladysheva_av@vector.nsc.ru
Россия, Кольцово

A. Gladysheva

State Scientific Center of Virology and Biotechnology «Vector»; Novosibirsk National Research State University

Email: gladysheva_aa@vector.nsc.ru
ORCID iD: 0000-0002-9490-1939

Graduate student, Assistant, Department of Molecular Virology for Flaviviruses and Viral Hepatitis

俄罗斯联邦, 630559, Novosibirsk region, Koltsovo; 630090, Novosibirsk

参考

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