MOLECULAR CHARACTERIZATION OF SRIVASTAVANEMA SP. FROM VIETNAMESE HAIRY-FOOTED FLYING SQUIRRELS BELOMYS PEARSONII (GRAY, 1842)
- Authors: Vlasenkov S.A.1, Balakirev A.E.1,2, Spiridonov S.E.1
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Affiliations:
- A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences
- Joint Russian-Vietnamese Tropical Research and Technology Center
- Issue: Vol 59, No 5 (2025)
- Pages: 352–361
- Section: Articles
- URL: https://journal-vniispk.ru/0031-1847/article/view/355718
- DOI: https://doi.org/10.7868/S3034586325050026
- ID: 355718
Cite item
Abstract
Heligmonellidae is a family of parasitic nematodes that includes parasites of tropical and subtropical mammals. Flying squirrels (Pteromyini, Sciuridae) are one of the characteristic hosts of heligmonellid nematodes. The nematodes of the genus Srivastavanema were collected from the flying squirrel Belomys pearsonii during a zoological expedition of the Joint Russian-Vietnamese Center for Tropical Research and Technology. Morphological study of the nematodes was carried out using optical and scanning electron microscopy. Partial sequences of 28S rRNA gene and ITS2 region were also obtained. Phylogenetic analysis based on 28S rRNA revealed related forms within Mexican rodent parasites. Further analysis of the molecular phylogeny with the inclusion of other loci is necessary to resolve the current controversy between classifications based on molecular and morphological data.
Keywords
About the authors
S. A. Vlasenkov
A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences
Email: svlasenkov22@gmail.com
Moscow, Russia
A. E. Balakirev
A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences; Joint Russian-Vietnamese Tropical Research and Technology Center
Email: svlasenkov22@gmail.com
Moscow, Russia; Hanoi, Vietnam
S. E. Spiridonov
A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences
Author for correspondence.
Email: svlasenkov22@gmail.com
Moscow, Russia
References
- Durette-Desset M.-C. 1971. Essai de classification des Nématodes Héligmosomes. Corrélation avec paléobiogéographie des hȏtes. Mémoires du Muséum national d’Histore naturelle, Paris, nouvelle série, Serie A, Zoologie 69: 1–126. [Durette-Desset M.-C. 1971. Attempt at classification of Heligmosomes Nematodes. Correlation with paleobiogeography of the hosts. Memoirs of the National Museum of Natural History, Paris, new series, Series A, Zoology 69: 1–126. (In French)].
- Durette-Desset M.-C. 1983 Keys to the genera of the Superfamily Trichostrongyloidea. In: Anderson R.S., Chabaud A.G. (eds), CIH Keys to the nematode parasites of vertebrates, Commonwealth Agricultural Bureau, 10: 1–68.
- Durette-Desset M.-C. 1985 Trichostrongylid nematodes and their vertebrate hosts: reconstruction of the phylogeny of a parasitic group. Advances in Parasitology 24: 239–306.
- Gasser R.B., Chilton N.B., Hoste H., Beveridge I. 1993. Rapid sequencing of rDNA from single worms and eggs of parasitic helminths. Nucleic acids research 21 (10): 2525–2526. https://doi.org/10.1093/nar/21.10.2525
- Holterman M., van der Wurff A., van den Elsen S., van Megen H., Bongers T., Holovachov O., Bakker J., Helder J. 2006. Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown Clades. Molecular Biology and Evolution 23: 1792–1800. https://doi.org/10.1093/molbev/msl044
- Miyamoto A., Anada M., Tsuchiya K., Nakama S., Yokohata Y. 2017. Srivastavanema musasabi (Nematoda, Heligmosomoidea) Obtained from Two Species of Flying Squirrels (Pteromys momonga and P. volans) in Japan, Japanese Journal of Zoo and Wildlife Medicine 22 (3): 51–54. https://doi.org/10.5686/jjzwm.22.51
- Nadler S.A., Hudspeth D.S.S. 2000. Phylogeny of the Ascaridoidea (Nematoda: Ascaridida) based on three genes and morphology: hypotheses of structural and sequence evolution. Journal of Parasitology 86 (2): 380–393. https://doi.org/10.1645/0022-3395(2000)086[0380:POTANA]2.0.CO;2
- Panti-May J.A., Moguel-Chin W.I., Hernndez-Mena D.I., Crdenas-Vargas M.H., Torres-Castro M., Garca-Prieto L., Digiani M.C., Hernndez-Betancourt S.F., Vidal-Martnez V.M. 2023. Helminths of small rodents (Heteromyidae and Cricetidae) in the Yucatan Peninsula, Mexico: an integrative taxonomic approach to their inventory. Zootaxa 5357 (2): 205–240. https://doi.org/10.11646/zootaxa.5357.2.3
- Rojas A., Germitsch N., Oren S. Sazmand A., Deak G. 2024. Wildlife parasitology: sample collection and processing, diagnostic constraints, and methodological challenges in terrestrial carnivores. Parasites Vectors 17: 127. https://doi.org/10.1186/s13071-024-06226-4
- Smith A.T., Xie Y. 2008. A Guide to the Mammals of China. Princeton, New Jersey, Princeton University Press. 576 p.
- Tamura K., Stecher G., Kumar S. 2021. MEGA11: Molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38: 3022–3027. https://doi.org/10.1093/molbev/msab120
- Thomas W.K., Vida J.T., Frisse L.M., Mundo M., Baldwin J.G. 1997. DNA sequences from formalin-fixed nematodes: integrating molecular and morphological approaches to taxonomy. Journal of Nematology 29 (3): 250–254.
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