Application of the New Degrader Strain Bacillus mobilis 34T for Soil Treatment from 2,4,5-Trichlorophenoxyacetic Acid

Аннотация

A 2,4,5-Trichlorophenoxyacetic acid (2,4,5-T)-degrading strain isolated from a soil sample of the largest producer of herbicides in Russia was described. The strain was identified as Bacillus mobilis 34Т according to the cultural, morphological, morphometric, physiological, and biochemical features as well as the results from comparative analysis of the 16S rRNA gene sequence. The growth of B. mobilis 34Т in the batch culture with using 2,4,5-T as a sole source of carbon and energy was studied. It was showed that 2,4,5-T content was reduced from the initial level by 29 and 62% during the first and ninth day, respectively. The strain was used for the treatment of a soil contaminated with 2,4,5-T. The decontamination degree was 41, 52, and 58% on the fifth, tenth, and 15th–20th days of cultivation, respectively.