Email this article Purification method optimization of recombinant basic fibroblast growth factor rhfgf-2 expressed in methylotrophic yeast Pichia pastoris
- Authors: Misterova A.A.1, Gerasimov A.S.1
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Affiliations:
- Vyatka State University
- Issue: Vol 61, No 4 (2025)
- Pages: 341-348
- Section: Articles
- URL: https://journal-vniispk.ru/0555-1099/article/view/353486
- DOI: https://doi.org/10.7868/S3034574
- ID: 353486
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Abstract
Basic human fibroblast growth factor FGF-2 plays a major role in wound healing. Thus, its regenerative potential attracts both researchers and commercial drug manufacturers, including cell therapy developers. This research aims to optimize purification method to obtain high purity rhFGF-2 expressed in methylotrophic yeast Pichia pastoris. Suggested method allows purification of rhFGF-2 with purity of >98% as determined by SDS-PAGE. The effective proliferative dose of rhFGF-2 determined by WST-1 proliferative assay on 3T3 mouse fibroblast cell culture is 5.73 ± 2.16 ng/ml. The presented optimized technique may be attractive for the development of an industrial technology for rhFGF-2 production.
About the authors
A. A. V. Misterova
Vyatka State University
Email: usr21438@vyatsu.ru
Kirov, 610000 Russia
A. S. Gerasimov
Vyatka State University
Author for correspondence.
Email: usr21438@vyatsu.ru
Kirov, 610000 Russia
References
- Zhang D., Mai Q., Li T., Huang J., Ding C., Jia M. et al. // Stem Cell Res. Ther. 2016. V. 7. № 1. https://doi.org/10.1186/s13287-016-0347-7
- Estapé D., Heuvel J.V.D., Rinas U. // Biochem. J. 1998. V. 335. № 2. P. 343–349.
- Chen R., John J., Lavrentieva A., Müller S., Tomala M., Zhao Y. et al. // Engineering in Life Sciences. 2012. V. 12. № 1. P. 29 –38.
- Mu X., Kong N., Chen W., Zhang T., Shen M., Yan W. // Protein Expr. Purif.. 2008. V. 59. № 2. P. 282–288.
- Sauer D.G., Mosor M., Frank A.C., Weiß F., Christler A., Walch N. et al. // Protein Expr. Purif.. 2019. V. 153. P. 70–82.
- Патент РФ . 2023. № 2804544.
- Garcia-Ortega X., Adelantado N., Ferrer P., Montesinos J.L., Valero F. // Process Biochemistry. 2016. V. 51. № 6. P. 681–691.
- Sauer D.G., Mosor M., Jungbauer A., Dürauer A. // Sep . Purif. Technol. 2021. V. 254. https://doi.org/10.1016/j.seppur.2020.117564
- Мистерова А.-А.В., Чичерин В.А., Герасимов А.С. // Прикл. биохимия и микробиология. 2023. T. 59. № 4. C. 383–391.
- Bolten S.N., Knoll A.S., Li Z., Gellermann P., Pepelanova I., Rinas U. et al. // Journal of Chromatography A. 2020. V. 1626. https://doi.org/10.1016/j.chroma.2020.461367
- Abdelhakim M., Lin X., Ogawa R. // Dermatol. Ther . (Heidelb.). 2020. V. 10. № 4. P. 569– 587.
- Kanemaru S. // Operative Techniques in Otolaryngology-Head and Neck Surgery. 2021. V. 32. № 2. P. 111–115.
- Nagayasu-Tanaka T., Anzai J., Takedachi M., Kitamura M., Harada T., Murakami S. // Regenerative Therapy. 2023. V. 23. P. 84–93.
- Fu X. Regenerative Medicine in China. / Ed. Fu X. Singapore: Springer Singapore, 2021. 485 p.
- Zheng K., Feng G., Zhang J., Xing J., Huang D., Lian M. et al. // Int. J. Neurosci. 2021. V. 131. № 7. P. 625–633.
- Moghtader F., Tabata Y., Karaöz E. // Gels. 2024. V. 10 . № 8. https://doi.org/10.3390/gels10080493
- Chen B., He J., Yang H., Zhang Q., Zhang L., Zhang X. et al. // Sci Rep. 2015. V. 5. № 1. https:// doi.org/10.1038/srep09017
- Kona S., Dong J.F., Liu Y., Tan J., Nguyen K.T. // Int. J. Pharm. 2012. V. 423. № 2. P. 516 –524.
- Auletta J.J., Zale E.A., Welter J.F., Solchaga L.A. // Stem Cells International. 2011. V . 3. https :// doi . org /10.4061/2011/235176
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