Optimization of Prochlorothrix hollandica cyanobacteria culturing for obtaining myristoleic acid
- Авторы: Lapina S.S.1, Maslova I.P.1, Pchelkin V.P.1, Bedbenov V.S.1, Mironov K.S.1, Zayadan B.K.2, Userbaeva A.A.2, Sinetova M.A.1, Los D.A.1
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Учреждения:
- Institute of Plant Physiology
- Faculty of Biology and Biotechnology
- Выпуск: Том 63, № 4 (2016)
- Страницы: 558-565
- Раздел: Research Papers
- URL: https://journal-vniispk.ru/1021-4437/article/view/179245
- DOI: https://doi.org/10.1134/S1021443716040087
- ID: 179245
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Аннотация
Plankton filament cyanobacteria Prochlorothrix hollandica is characterized by a very high content of C14 and C16 fatty acids (FA) in the lipid membranes. Depending on culturing conditions of the cyanobacteria, total concentrations of myristic and myristoleic acids can reach 35% and those of palmitic and palmitoleic acids can reach 60% of all esterified FA cells. In P. hollandica, a variety of monounsaturated FA is represented by myristoleic and palmitic acids, and by hexadecenoic (C16:1) acid with olefin bond of cis-configuration, located in the Δ4 position. The process of intensive culturing for P. hollandica cells to yield a maximal biomass in order to isolate the pure drug of myristoleic acid derivative has been optimized. The use of a threestage purification gives 30 mg of chromatographically pure myristoleic acid methyl ester from 17 g of P. hollandica raw biomass (dry mass is 3 g), which is 1% of dry cell mass.
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Об авторах
S. Lapina
Institute of Plant Physiology
Email: losda@ippras.ru
Россия, ul. Botanicheskaya 35, Moscow, 127276
I. Maslova
Institute of Plant Physiology
Email: losda@ippras.ru
Россия, ul. Botanicheskaya 35, Moscow, 127276
V. Pchelkin
Institute of Plant Physiology
Email: losda@ippras.ru
Россия, ul. Botanicheskaya 35, Moscow, 127276
V. Bedbenov
Institute of Plant Physiology
Email: losda@ippras.ru
Россия, ul. Botanicheskaya 35, Moscow, 127276
K. Mironov
Institute of Plant Physiology
Email: losda@ippras.ru
Россия, ul. Botanicheskaya 35, Moscow, 127276
B. Zayadan
Faculty of Biology and Biotechnology
Email: losda@ippras.ru
Казахстан, pr. Al-Farabi 71, Almaty
A. Userbaeva
Faculty of Biology and Biotechnology
Email: losda@ippras.ru
Казахстан, pr. Al-Farabi 71, Almaty
M. Sinetova
Institute of Plant Physiology
Email: losda@ippras.ru
Россия, ul. Botanicheskaya 35, Moscow, 127276
D. Los
Institute of Plant Physiology
Автор, ответственный за переписку.
Email: losda@ippras.ru
Россия, ul. Botanicheskaya 35, Moscow, 127276
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