Determination of proinflammatory cytokine levels to assess the reparative potential of tissues in ischemic damage and local correction with hyaluronic acid
- Authors: Shperling M.I.1, Kubyshkin A.V.1, Uryupin K.I.2
-
Affiliations:
- V. Vernadsky Crimean Federal University
- Rostov State Medical University
- Issue: Vol 27, No 4 (2024)
- Pages: 899-906
- Section: SHORT COMMUNICATIONS
- URL: https://journal-vniispk.ru/1028-7221/article/view/267884
- DOI: https://doi.org/10.46235/1028-7221-16861-DOP
- ID: 267884
Cite item
Full Text
Abstract
The hypothesis of the study was the assumption that local correction of secondary alteration stops excessive inflammation activity and promotes early activation of reparative processes. Objective: in an animal experiment, to evaluate the dynamics in blood plasma of the concentration of circulating inflammatory cytokines in acute ischemic damage to soft tissues and after local action of a hyaluronic acid-based drug.
In anesthetized Wistar rats, acute ischemic damage to the soft tissues of the thigh was simulated by mechanical compression with a vise for 7 hours. After removing the vise, the rats were divided into 2 groups of 32 individuals: control (without treatment) and experimental (3 hours after removing the vise, the drug was injected into the area bordering the compression zone with a sterile syringe based on hyaluronic acid “Hyalift-3.5”, diluted with 0.9% solution to the final concentration of the drug – 1.75%). The concentration of cytokines in blood plasma was determined, histological sections of soft tissues in the compression area were examined.
An increase in the plasma concentration of proinflammatory cytokines (IL-1β, TNFα, IL-6) was revealed during the entire follow-up period (21 days), especially 7 days after the injury. To the greatest extent, these changes were observed in animals in the control group, which was manifested by a statistically significant increase in the concentration of IL-1β, TNFα and IL-6, relative to the values in intact rats: on average by 7.2; 3.7 and 5.3 times, respectively. In rats in the experimental group, local administration of a hyaluronic acid-based drug already in the early period after injury limited the severity of inflammation (an increased concentration of IL-10 was recorded in blood plasma).
The sanogenetic role of local administration of hyaluronic acid in the wound process is mediated by the relief of inflammation in the early period of damage and is realized in increasing the reparative potential of tissues according to the variant of earlier and significant formation of musculoskeletal tissue regenerates.
Full Text
##article.viewOnOriginalSite##About the authors
M. I. Shperling
V. Vernadsky Crimean Federal University
Email: kubyshkin_av@mail.ru
Postgraduate Student, Department of General and Clinical Pathophysiology, Order of Labor Red Banner S. Georgievsky Medical Institute
Russian Federation, Simferopol, Republic of CrimeaA. V. Kubyshkin
V. Vernadsky Crimean Federal University
Author for correspondence.
Email: kubyshkin_av@mail.ru
PhD, MD (Medicine), Professor, Head, Department of General and Clinical Pathophysiology, Order of Labor Red Banner S. Georgievsky Medical Institute
Russian Federation, Simferopol, Republic of CrimeaK. I. Uryupin
Rostov State Medical University
Email: kubyshkin_av@mail.ru
Student, Pediatric Faculty
Russian Federation, Rostov-on-DonReferences
- Анисимова Л.В., Харченко В.З., Федосов М.И., Кубышкин А.В. Система протеолиза сыворотки крови в формировании синдрома полиорганной недостаточности // Крымский журнал экспериментальной и клинической медицины, 2016. Т. 6, № 1. С. 4-7. [Anisimova L.V., Kharchenko V.Z., Fedosov M.I., Kubyshkin A.V. The role of proteinase-inhibitory system of blood serum in formation of the syndrome of polyorgan insufficiency. Krymskiy zhurnal eksperimentalnoy i klinicheskoy meditsiny = Crimea Journal of Experimental and Clinical Medicine, 2016, Vol. 6, no. 1, pp. 4-7. (In Russ.)]
- Капулер О.М., Галеева А.Г., Сельская Б.Н., Камилов Ф. Гиалуронан: свойства и биологическая роль // Врач, 2015. № 2. С. 25-27. [Kapuler O.M., Galeeva A.G., Selskaya B.N., Kamilov F. Hyaluronan: properties and biological role. Vrach = Doctor, 2015, no. 2, pp. 25-27. (In Russ.)]
- Костина О.В., Преснякова М.В., Кузнецова В.Л. Особенности воспалительного ответа у пациентов с острыми и длительно незаживающими ожоговыми ранами // Клиническая лабораторная диагностика, 2017. Т. 62, № 7. С. 410-413. [Kostina O.V., Presnyakova M.V., Kuznetsova V.L. The characteristics of inflammatory response in patients with acute and persistent non-healing burn wounds. Klinicheskaya laboratornaya diagnostika = Russian Clinical Laboratory Diagnostics, 2017, Vol. 62, no. 7, pp. 410-413. (In Russ.)]
- Потапнев М.П. Иммунные механизмы стерильного воспаления // Иммунология, 2015. Т. 36, № 5. С. 312-318. [Potapnev M.P. Immune mechanisms of sterile inflammation. Immunologiya = Immunologiya, 2015, Vol. 36, no. 5, pp. 312-318. (In Russ.)]
- Серебренникова С.Н., Семинский И.Ж., Гузовская Е.В., Гуцол Л.О. Воспаление – фундаментальный патологический процесс: лекция 1 (альтерация, сосудистые реакции) // Байкальский медицинский журнал, 2023. Т. 2, № 2. С. 53-64. [Serebrennikova S.N., Seminsky I.Zh., Guzovskaiia E.V., Gutsol L.O. Inflammation as a fundamental pathological process: lecture 1 (alteration, vascular reactions). Bajkalskiy medicinskiy zhurnal = Baikal Medical Journal, 2023, Vol. 2, no 2, pp. 53-64. (In Russ.)]
- Супильников А.А., Девяткин А.А., Павлова О.Н., Гуленко О.Н. Морфологические и физиологические аспекты течения раневого процесса (литературный обзор) // Вестник медицинского института «РЕАВИЗ», 2016. № 3. С. 144-151. [Supil’nikov A.A., Devyatkin A.A., Pavlova O.N., Gulenko O.N. The morphology of the wound healing process (literature review). Vestnik meditsinskogo instituta “REAVIZ” = Bulletin of the Medical Institute “REAVIZ”: Rehabilitation, Doctor and Health. 2016, no. 3, pp. 144-151. (In Russ.)]
- Шперлинг И.А., Шулепов А.В., Шперлинг Н.В., Юркевич Ю.В., Лютов Р.В., Арутюнян А.А., Кузьмина О.Ю. Ранняя локальная коррекция микроциркуляторных и метаболических нарушений при экспериментальной травматической ишемии мышц // Политравма, 2021. № 2. С. 94-102. [Shperling I.A., Shulepov A.V., Shperling N.V., Yurkevich Yu.V., Lutov R.V., Arutyunyan A.A., Kuzmina O.Yu. Early local correction of microcirculatory and metabolic disorders in experimental traumatic muscle ischemia. Politravma = Polytrauma, 2021, no. 2, pp. 94-102. (In Russ.)]
- Шперлинг И.А., Шулепов А.В., Шперлинг Н.В., Юркевич Ю.В., Кузьмина О.Ю., Арутюнян А.А., Заргарова Н.И. Саногенетические и фармакологические эффекты локального применения гиалуроновой кислоты при экспериментальной компрессионной травме мягких тканей // Крымский журнал экспериментальной и клинической медицины, 2020. Т. 10, № 2. С. 53-60. [Shperling I.A., Shulepov A.V., Shperling N.V., Yurkevich Yu.V., Kuz’mina O.Yu., Arutyunyan A.A., Zargarova N.I. Sanogenetic and pharmacological effects of local application of Hyaluronic acid in experimental soft tissue compression trauma. Krymskiy zhurnal eksperimentalnoy i klinicheskoy meditsiny = Crimea Journal of Experimental and Clinical Medicine, 2020, Vol. 10, no. 2, pp. 53-60. (In Russ.)]
- Aya K.L., Stern R. Hyaluronan in wound healing: rediscovering a major player. Wound Repair Regen., 2014, Vol. 22, no 5, pp. 579-593.
- Fallacara A., Baldini E., Manfredini S., Vertuani S. Hyaluronic acid in the third millennium. Polymers (Basel), 2018, Vol. 10, no. 7, pp. 701-710.
- Johnson P., Arif A.A., Lee-Sayer S.S.M., Dong Y. Hyaluronan and its interactions with immune cells in the healthy and inflamed lung. Front. Immunol., 2018, Vol. 9, pp. 2787-2795. doi: 10.3389/fimmu.2018.02787.
- Lee C.H., Chiang C.F., Kuo F.C., Su S.C., Huang C.L., Liu J.S., Lu C.H., Hsieh C.H., Wang C.C., Lee C.H., Shen P.H. Affiliations High-molecular-weight hyaluronic acid inhibits il-1β-induced synovial inflammation and macrophage polarization through the GRP78-NF-κB signaling pathway. Int. J. Mol. Sci., 2021, Vol. 22, no. 21, 11917. doi: 10.3390/ijms222111917.
- Murata I., Miyake Y., Takahashi N., Suzuki R., Fujiwara T., Sato Y., Inoue Y., Kobayashi J., Kanamoto I. Low-dose sodium nitrite fluid resuscitation prevents lethality from crush syndrome by improving nitric oxide consumption and preventing myoglobin cytotoxicity in kidney in a rat model. Shock. 2017, Vol. 48, no. 1, pp. 112-118.
- Pan S.C., Wu L.W., Chen C.L., Shieh S.J., Chiu H.Y. Angiogenin expression in burn blister fluid: implications for its role in burn wound neovascularization. Ann. Plast. Surg., 2018, Vol. 2, no. 80 (2S Suppl. 1), pp. 79-83.
- Wight T.N. Provisional matrix: A role for versican and hyaluronan. Matrix Biol., 2017, Vol. 60-61, no. 7, pp. 38-56.
Supplementary files
