An atomic-force-microscopy study of the structure of surface layers of intact fibroblasts


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

Intact embryonic fibroblasts on a collagen-treated substrate have been studied by atomic-force microscopy (AFM) using probes of two types: (i) standard probes with tip curvature radii of 2–10 nm and (ii) special probes with a calibrated 325-nm SiO2 ball radius at the tip apex. It is established that, irrespective of probe type, the average maximum fibroblast height is on a level of ~1.7 μm and the average stiffness of the probe–cell contact amounts to ~16.5 mN/m. The obtained AFM data reveal a peculiarity of the fibroblast structure, whereby its external layers move as a rigid shell relative to the interior and can be pressed inside to a depth dependent on the load only.

Авторлар туралы

M. Khalisov

Pavlov Institute of Physiology; St. Petersburg National Research University of Information Technologies

Email: alexander.ankudinov@mail.ioffe.ru
Ресей, St. Petersburg, 199034; St. Petersburg, 197101

A. Ankudinov

St. Petersburg National Research University of Information Technologies; Ioffe Physical Technical Institute

Хат алмасуға жауапты Автор.
Email: alexander.ankudinov@mail.ioffe.ru
Ресей, St. Petersburg, 197101; St. Petersburg, 194021

V. Penniyaynen

Pavlov Institute of Physiology

Email: alexander.ankudinov@mail.ioffe.ru
Ресей, St. Petersburg, 199034

I. Nyapshaev

Ioffe Physical Technical Institute

Email: alexander.ankudinov@mail.ioffe.ru
Ресей, St. Petersburg, 194021

A. Kipenko

Pavlov Institute of Physiology; Federal Almazov Northwest Medical Research Centre

Email: alexander.ankudinov@mail.ioffe.ru
Ресей, St. Petersburg, 199034; St. Petersburg, 197341

K. Timoshchuk

St. Petersburg National Research University of Information Technologies

Email: alexander.ankudinov@mail.ioffe.ru
Ресей, St. Petersburg, 197101

S. Podzorova

Pavlov Institute of Physiology

Email: alexander.ankudinov@mail.ioffe.ru
Ресей, St. Petersburg, 199034

B. Krylov

Pavlov Institute of Physiology

Email: alexander.ankudinov@mail.ioffe.ru
Ресей, St. Petersburg, 199034

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