Chiral chromatographic analysis of amino acids with pre-column derivatization by o-phthalaldehyde: improving the determination of enantiomers using ion-pair reagents
- Авторлар: Panin N.V.1, Pirogov I.V.1, Guranda D.T.1, Švedas V.K.1
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Мекемелер:
- Lomonosov Moscow State University
- Шығарылым: Том 17, № 4 (2025)
- Беттер: 103-109
- Бөлім: Research Articles
- URL: https://journal-vniispk.ru/2075-8251/article/view/365064
- DOI: https://doi.org/10.32607/actanaturae.27703
- ID: 365064
Дәйексөз келтіру
Аннотация
The development of effective and accessible methods for the chiral analysis of amino acids is an important scientific and practical necessity. One of the most common and convenient techniques is the chromatographic determination of individual enantiomers of amino acids with preliminary conversion of enantiomers into diastereomers, which can then be separated on conventional achiral columns. We have shown that by adding ion-pair reagents to the eluent and varying their structure, one can regulate the efficiency of a chiral amino acid analysis based on the chromatographic determination and resolution of the diastereomeric isoindoles obtained upon pre-column derivatization of amino acids by o-phthalaldehyde in the presence of N-acetyl-L-cysteine. The use of ion-pair reagents allows one to achieve a better resolution of diastereomeric isoindole peaks, while the analysis time can be reduced by increasing the ionic strength. Hence, adding ion-pair reagents and optimizing the mobile phase composition are useful approaches in the engineering of an amino acid chiral analysis, along with the synthesis of new chiral SH compounds and the choice of stationary phases.
Авторлар туралы
Nikolay Panin
Lomonosov Moscow State University
Email: panin@belozersky.msu.ru
Belozersky Institute of Physicochemical Biology
Ресей, Moscow, 119234Ivan Pirogov
Lomonosov Moscow State University
Email: ikaesovich@mail.ru
Faculty of Chemistry
Ресей, Moscow, 119234Dorel Guranda
Lomonosov Moscow State University
Email: dorel@belozersky.msu.ru
Belozersky Institute of Physicochemical Biology
Ресей, Moscow, 119234Vytas Švedas
Lomonosov Moscow State University
Хат алмасуға жауапты Автор.
Email: vytas@belozersky.msu.ru
Belozersky Institute of Physicochemical Biology; Faculty of Bioengineering and Bioinformatics
Ресей, Moscow, 119234Әдебиет тізімі
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