Obtaining and using erythrocyte antigenic meliidosis diagnostic agent in the analysis of serum samples from meliidosis-endemic provinces Ha Giang, Lang Son and Quang Ninh of the Socialist Republic of Vietnam
- Authors: Tereshko D.L.1, Novitskaya I.V.1, Zakharova I.B.1, Trien D.2, Kuznetsov A.N.2, Kulakov M.Y.1, Budchenko A.A.1, Pushkar V.G.1, Viktorov D.V.1, Toporkov A.V.1
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Affiliations:
- Volgograd Plague Control Research Institute, Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
- Joint Russian-Vietnamese Tropical Research and Technology Center
- Issue: Vol 12, No 5 (2022)
- Pages: 919-928
- Section: ORIGINAL ARTICLES
- URL: https://journal-vniispk.ru/2220-7619/article/view/119154
- DOI: https://doi.org/10.15789/2220-7619-OAU-1933
- ID: 119154
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Abstract
Melioidosis is a particularly dangerous infection with endemic distribution caused by the Gram-negative microorganism from the pathogenicity group II Burkholderia pseudomallei. In endemic countries, melioidosis holds one of the leading places in mortality rate after HIV, tuberculosis and, in recent years, COVID-19. The natural ecological pathogen niches are located in tropical and subtropical climate zones, primarily in Southeast Asia and Australia, where its existence as a species is maintained in moist soil and water in a certain temperature environmental range. However, at present, more and more often cases of melioidosis are registered outside endemic territories, which emphasizes the relevance of improving the means and methods of laboratory diagnostics of this disease both for countries located in the zone of natural foci as well as for local healthcare of the countries after importation of this poorly known infection into their territory. In such countries, including the Russian Federation, the population has no natural immunity to the pathogen, and therefore this infection acquires even greater clinical and epidemiological significance. In the Volgograd Plague Control Research Institute, an erythrocyte antigenic melioidosis diagnostic agent for IHA was designed allowing to detect the presence of serum melioidosis antibodies. The diagnostic agent was obtained on the basis of a biological carrier — ram erythrocytes sensitized with isolated protein antigenic complexes of B. pseudomallei. The high analytical characteristics of the diagnostic agent were confirmed on sera models of immunized and recovering experimental animals. Using the obtained set of reagents, the level of serum antibodies against the causative agent of melioidosis was studied in residents from the 3 provinces of Vietnam (Ha Giang, Lang Son and Quang Ninh), as well as in control group composed of residents of the Volgograd region. In samples obtained from a non-endemic region, not more than 25% of cases contained IHA titers at lower than 1:10 dilution, which is apparently due to cross-reactivity of serum immunoglobulins. Positive serum samples from clinically healthy residents of Ha Giang, Lang Son and Quang Ninh provinces were at a titer of 1:10 detected in 71.5%, in dilutions of 1:20–1:80 — in 28.5% of cases. Thus, we believe that serum antibody titer of 1:80 established in the IHA results, has a diagnostic significance, reflecting the intensity of the anti-melioidosis populational immunity.
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##article.viewOnOriginalSite##About the authors
D. L. Tereshko
Volgograd Plague Control Research Institute, Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Author for correspondence.
Email: Dltereshko@gmail.com
Researcher, Laboratory of Immunodiagnostic Preparations
Russian Federation, VolgogradI. V. Novitskaya
Volgograd Plague Control Research Institute, Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Email: irvnov@mail.ru
PhD (Medicine), Associate Professor, Leading Researcher, Head of the Department of Immunology and Experimental Production of Medical Immunobiological Preparations
Russian Federation, VolgogradI. B. Zakharova
Volgograd Plague Control Research Institute, Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Email: zib279@gmail.com
PhD (Biology), Associate Professor, Head of the Department of Microbiology
Russian Federation, VolgogradD. Trien
Joint Russian-Vietnamese Tropical Research and Technology Center
Email: tropcenterhanoi@mail.ru
co-director
Viet Nam, HanoiA. N. Kuznetsov
Joint Russian-Vietnamese Tropical Research and Technology Center
Email: forestkuz@mail.ru
PhD, MD (Biology), Сo-director
Viet Nam, HanoiM. Ya. Kulakov
Volgograd Plague Control Research Institute, Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Email: mihailkulakov2115@gmail.com
PhD (Medicine), Senior Researcher, Laboratory of Immunodiagnostic Preparations
Russian Federation, VolgogradA. A. Budchenko
Volgograd Plague Control Research Institute, Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Email: budchenko1976@yandex.ru
PhD (Biology), Senior Researcher, Proteomic Analysis Laboratory
Russian Federation, VolgogradV. G. Pushkar
Volgograd Plague Control Research Institute, Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Email: gunner50@mail.ru
PhD (Biology), Senior Researcher, Laboratory of Immunodiagnostic Preparations
Russian Federation, VolgogradD. V. Viktorov
Volgograd Plague Control Research Institute, Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Email: dvictorov09@gmail.com
PhD, MD (Biology), Associate Professor, Deputy Director
Russian Federation, VolgogradA. V. Toporkov
Volgograd Plague Control Research Institute, Federal Service for Surveillance in the Sphere of Consumers Rights Protection and Human Welfare
Email: topotkov-andreyy@rambler.ru
PhD, MD (Medicine), Director
Russian Federation, VolgogradReferences
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