Metabolic activity of peripheral blood neutrophils in patients with Opisthorchis felineus invasion and skin syndrome
- 作者: Tsukanov V.V.1, Gorchilova E.G.2, Kolenchukova O.A.1, Gvozdev I.I.1, Savchenko A.A.1, Vasyutin A.V.1, Tonkikh J.L.1, Rzhavicheva O.S.2, Borisov A.G.1
-
隶属关系:
- Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS
- Private Healthcare Institution Clinical hospital “RZD-Medicine” of Krasnoyarsk City
- 期: 卷 11, 编号 3 (2021)
- 页面: 570-576
- 栏目: SHORT COMMUNICATIONS
- URL: https://journal-vniispk.ru/2220-7619/article/view/119009
- DOI: https://doi.org/10.15789/2220-7619-MAO-1415
- ID: 119009
如何引用文章
全文:
详细
The aim was to examine functional activity of peripheral blood neutrophilic granulocytes in patients with Opisthorchis felineus invasion and skin syndrome. Materials and methods. A total of 92 patients with chronic opisthorchiasis were examined, of which 38 patients (20 males and 18 females, average age 35.7±3.9 years) and 54 patients (28 males and 26 females, average age 36.5±4.1 years) with/without skin syndrome, as well as 32 apparently healthy subjects (17 males and 15 females, average age 41.5 years). Detection of adult parasite eggs or bodies in the duodenal contents and/or feces was used as the major diagnostic method of opisthorchiasis used in all 92 opisthorchiasis patients. Liver fibrosis was assessed by elastometry method according to the METAVIR scale in all 92 opisthorchiasis patients. Functional activity of peripheral blood neutrophilic granulocytes was examined in all 92 opisthorchiasis patients and 32 healthy individuals from the control group by chemiluminescent analysis measuring intensity of reactive oxygen species production in spontaneous and zymosan-induced reactions in lucigenin and luminol-dependent processes. Results. Opisthorchiasis patients with vs. without skin syndrome suffered at higher rate from pain in the right hypochondrium, dyspepsia, astheno-vegetative syndrome, skin itching, blood eosinophilia, hyperbilirubinemia, cholestatic syndrome, cholecystitis, hepatomegaly, and had elevated serum immunoglobulin E level. Liver fibrosis F3—F4 according to METAVIR was determined in 18.4% and 11.1% of opisthorchiasis patients with vs. without skin syndrome (OR = 1.78; CI 0.57—5.57; p = 0.05). Both luminol- and lucigenin-dependent processes in opisthorchiasis patients with vs. without skin syndrome revealed significantly decreased functional activity of neutrophilic granulocytes which was evidenced by significantly decreased maximum intensity of reactive oxygen species production and the area under the chemiluminescence curve both in spontaneous and zymosan-induced reaction. Conclusion. The results suggest that the presence of skin syndrome in patients with opisthorchiasis is accompanied by more prominent clinical and laboratory manifestations, decreased functional activity of peripheral blood neutrophils, thereby allowing to consider it as a marker of intense pathology.
作者简介
V. Tsukanov
Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS
编辑信件的主要联系方式.
Email: gastro@impn.ru
ORCID iD: 0000-0002-9980-2294
Vladislav V. Tsukanov - PhD, MD (Medicine), Professor, Head of the Clinical Department of the Digestive System Pathology of Adults and Children, Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS.
660022, Krasnoyarsk, Partizan Zheleznyak str., 3-G.
Phone: +7 (391) 256-81-71; Fax: +7 (391) 228-06-83
SPIN: 2529-9513
俄罗斯联邦E. Gorchilova
Private Healthcare Institution Clinical hospital “RZD-Medicine” of Krasnoyarsk City
Email: yourself.15@mail.ru
Gastroenterologist, Gastroenterological Department, Private Healthcare Institution Clinical hospital “RZD-Medicine” of Krasnoyarsk City.
Krasnoyarsk.
俄罗斯联邦O. Kolenchukova
Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS
Email: kalina-chyikova@mail.ru
ORCID iD: 0000-0001-9552-447X
PhD, MD (Biology), Leading Researcher, Laboratory of Molecular and Cellular Physiology and Pathology, Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS.
Krasnoyarsk.
SPIN-код: 8008-5580
俄罗斯联邦I. Gvozdev
Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS
Email: leshman-mult@mail.ru
Junior Researcher, Laboratory of Molecular and Cellular Physiology and Pathology, Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS.
Krasnoyarsk.
SPIN-код: 6203-4651
俄罗斯联邦A. Savchenko
Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS
Email: aasavchenko@yandex.ru
PhD, MD (Medicine), Professor, Head of the Laboratory of Molecular and Cellular Physiology and Pathology, Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS.
Krasnoyarsk.
SPIN-код: 3132-8260
俄罗斯联邦A. Vasyutin
Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS
Email: alexander@kraslan.ru
ORCID iD: 0000-0002-6481-3196
PhD (Medicine), Senior Researcher, Clinical Department of the Digestive System Pathology of Adults and Children, Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS.
Krasnoyarsk.
SPIN-код: 4075-4538
俄罗斯联邦J. Tonkikh
Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS
Email: tjulia@bk.ru
ORCID iD: 0000-0001-7518-1895
PhD (Medicine), Leading Researcher, Clinical Department of the Digestive System Pathology of Adults and Children, Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS.
Krasnoyarsk.
SPIN-код: 3292-9128
俄罗斯联邦O. Rzhavicheva
Private Healthcare Institution Clinical hospital “RZD-Medicine” of Krasnoyarsk City
Email: ros315@yandex.ru
PhD (Medicine), Head of the Gastroenterological Department, Private Healthcare Institution Clinical hospital “RZD-Medicine” of Krasnoyarsk City.
Krasnoyarsk.
俄罗斯联邦A. Borisov
Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS
Email: 2410454@mail.ru
ORCID iD: 0000-0002-9026-2615
PhD (Medicine), Leading Researcher, Laboratory of Molecular and Cellular Physiology and Pathology, Research Institute of Medical Problems of the North, Federal Research Center KSC SB RAS.
Krasnoyarsk.
SPIN-код: 9570-2254
俄罗斯联邦参考
- Мордвинов В.А., Фурман Д.П. «Обская болезнь» — недооцененная опасность // Наука в России. 2013. Т. 195, № 3. С. 15—24.
- Цуканов В.В., Селиверстова Е.В., Догадин С.А. Показатели липидного состава сыворотки крови и желчи при заболеваниях желчевыводящих путей у больных сахарным диабетом. Терапевтический архив. 2005. Т. 77, № 2. С. 15— 18.
- Цуканов В.В., Тонких Ю.Л., Бронникова Е.П., Манчук В.Т. Механизм нормолипидемии у северных народностей. Клиническая медицина. 1999. Т. 77, № 2. С. 38-39.
- Цуканов В.В., Тонких Ю.Л., Гилюк А.В., Васютин А.В., Коленчукова О.А., Ржавичева О.С., Борисов А.Г., Савченко А.А., Масленникова Н.А. Диагностика, клиника и лечение описторхоза. Доктор.Ру. 2019. № 8 (163). С. 49-53. doi: 10.31550/1727-2378-2019-163-8-49-53
- Antoniades C.G., Berry P.A., Wendon J.A., Vergani D. The importance of immune dysfunction in determining outcome in acute liver failure. J. Hepatol, 2008, vol. 49, no. 5, pp. 845—861. doi: 10.1016/j.jhep.2008.08.009
- Fedorova O.S., Janse J.J., Ogorodova L.M., Fedotova M.M., Achterberg R.A., Verweij J.J., Fernandez-Rivas M., Versteeg S.A., Potts J., Minelli C., van Ree R., Burney P., Yazdanbakhsh M. Opisthorchis felineus negatively associates with skin test reactivity in Russia-EuroPrevall-International Cooperation study. Allergy, 2017, vol. 72, no. 7, pp. 1096—1104. doi: 10.1111/all.13120
- Fedorova O.S., Kovshirina Y.V., Kovshirina A.E., Fedotova M.M., Deev I.A., Petrovskiy F.I., Filimonov A.V., Dmitrieva A.I., Kudyakov L.A., Saltykova I.V., Odermatt P., Ogorodova L.M. Opisthorchis felineus infection and cholangiocarcinoma in the Russian Federation: a review ofmedical statistics. Parasitol. Int, 2017, vol. 66, no. 4,pp. 365—371. doi: 10.1016/j.parint.2016.07.010
- Kubes P., Mehal W.Z. Sterile inflammation in the liver. Gastroenterology, 2012, vol. 143, no. 5, pp. 1158—1172. doi: 10.1053/j.gastro.2012.09.008
- Phathammavong O., Ali M., Phengsavanh A., Xaysomphou D., Odajima H., Nishima S., Kuroiwa C. Prevalence and potential risk factors of rhinitis and atopic eczema among schoolchildren in Vientiane capital, Lao PDR: ISAAC questionnaire. Biosci. Trends., 2008, vol. 2, no. 5, pp. 193—199.
- Pillay J., Kamp V.M., van Hoffen E., Visser T., Tak T., Lammers J.W., Ulfman L.H., Leenen L.P., Pickkers P., Koenderman L. A subset of neutrophils in human systemic inflammation inhibits T cell responses through Mac-1. J. Clin. Invest., 2012, vol. 122, no. 1, pp. 327-336. doi: 10.1172/JCI57990
- Poynard T., Bedossa P., Opolon P. Natural history of liver fibrosis progression in patients with chronic hepatitis C. The OBSVIRC, METAVIR, CLINIVIR, and DOSVIRC groups. Lancet, 1997, vol. 349, no. 9055, pp. 825-832. doi: 10.1016/s0140-6736(96)07642-8
- Stanghellini V., Chan F.K., Hasler W.L., Malagelada J.R., Suzuki H., Tack J., Talley N.J. Gastroduodenal disorders. Gastroenterology, 2016, vol. 150, no. 6, pp. 1380-1392. doi: 10.1053/j.gastro.2016.02.011
- Xu R., Huang H., Zhang Z., Wang F.S. The role of neutrophils in the development of liver diseases. Cell. Mol. Immunol., 2014, vol. 11, no. 3, pp. 224-231. doi: 10.1038/cmi.2014.2
- Zou Z., Li B., Xu D., Zhang Z., Zhao J.M., Zhou G., Sun Y., Huang L., Fu J., Yang Y., Jin L., Zhang W., Zhao J., Sun Y., Xin S., Wang F.S. Imbalanced intrahepatic cytokine expression of interferon-gamma, tumor necrosis factor-alpha, and interleukin-10 in patients with acute-on-chronic liver failure associated with hepatitis B virus infection. J. Clin. Gastroenterol., 2009, vol. 43, no. 2, pp. 182-190. doi: 10.1097/MCG.0b013e3181624464
补充文件
