The influence of bacteria of the genus Bacillus on the morphological parameters and photosynthetic apparatus of Allium cepa
- Авторлар: Kastornov A.A.1, Petrov S.A.1, Subbotin A.M.1
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Мекемелер:
- Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences
- Шығарылым: Том 17, № 4 (2025)
- Беттер: 45-63
- Бөлім: Plant Physiology and Biochemistry
- ##submission.datePublished##: 31.10.2025
- URL: https://journal-vniispk.ru/2658-6649/article/view/351917
- DOI: https://doi.org/10.12731/2658-6649-2025-17-4-1321
- ID: 351917
Дәйексөз келтіру
Толық мәтін
Аннотация
Background. The cryosphere is a repository of ancient ecosystems, in particular dispersed soils that have become frozen. It is known that these soils contain a certain amount of both abiotic and biotic components, including bacterial cells in a hypometabolic state with a content of about 104-106 CFU per gram of soil. Permafrost is widespread in the northern hemisphere, and its age ranges from hundreds of thousands to millions of years, so the bacterial components they contain can be fully attributed to paleobacteria. Modern flora associations with arctic paleobacteria are of interest not only from the point of researching view the fundamental principles of interaction between various organisms, but also as a promising biotechnological resource in adaptive plant growing.
Purpose. To assess the influence of arctic paleobacteria of the genus Bacillus on the morphophysiological parameters of the roots and feathers of the onion Allium cepa, as well as its photosynthetic activity at different cultivation temperatures.
Materials and methods. The object of the study was the onion Allium cepa, since this plant is sensitive to various environmental factors and is a standard for studying toxicity. We used bacteria of the genus Bacillus (strain 875 TS), registered in the Russian National Collection of Industrial Microorganisms under registration number B-12242.
The following morphophysiological parameters were assessed: the number of roots and feathers, their length and weight, the number of dark and curved roots and feathers, the concentration of photosynthesis pigments in the green part of Allium cepa seedlings using absorption spectrophotometry.
Results. The conducted research have shown that bacteria of the genus Bacillus strain 875 TS from permafrost rocks in a concentration of 104 at an incubation temperature of 36 ° C have the greatest positive effect on the content of photosynthetic pigments, root provision and development of the vegetative part of the onion Allium cepa.
Conclusion. Bacteria of the genus Bacillus influence plant development, in particular, the formation of the root and vegetative apparatus, enrich plants with useful substances and are a promising biological resource for the development of biopreparations to increase the yield of agricultural crops.
Авторлар туралы
Aleksandr Kastornov
Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: AlexKastornov@yandex.ru
ORCID iD: 0000-0002-0116-5291
SPIN-код: 7133-1500
Junior Research Scientist of Department of Bioresources Cryosphere
Ресей, 86, Malygina Str., Tyumen, 625026, Russian FederationSergei Petrov
Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences
Email: tumiki@yandex.ru
ORCID iD: 0000-0002-1566-2299
SPIN-код: 8382-8583
Doctor of Medicine, Professor, Principal Researcher of Department of Bioresources Cryosphere
Ресей, 86, Malygina Str., Tyumen, 625026, Russian FederationAndrey Subbotin
Federal Research Centre Tyumen Scientific Centre of Siberian Branch of the Russian Academy of Sciences
Email: subbotin.prion@yandex.ru
ORCID iD: 0000-0002-5135-3194
SPIN-код: 9865-7586
Cand. Sc. Biology, Assistant Professor, leading research scientist of Department of Bioresources Cryosphere
Ресей, 86, Malygina Str., Tyumen, 625026, Russian FederationӘдебиет тізімі
- Bazin, A. S., Subbotin, A. M., Petrov, S. A., Narushko, M. V., & Kalenova, L. F. (2021). Effect of drilling cuttings with different purification degrees on morphophysiological parameters of cereal plants. In Proceedings of the international conference “Modern studies of cryosphere transformation and issues of geotechnical safety of structures in the Arctic” (pp. 43–46). Salekhard. https://doi.org/10.7868/9785604610848009. EDN: https://elibrary.ru/YTRKWN
- Petrov, S. A., Enoktaeva, O. V., Subbotin, A. M., & Malchevsky, V. A. (2015). Comprehensive scoring system for assessing the impact of microorganisms on cell proliferation of Allium cepa L. root meristem. Fundamental Research, 74, 687–690. EDN: https://elibrary.ru/UGAQNJ
- Landina, L. N. (2021). Standardization of dry extract of pumpkin pulp juice with hypolipidemic effect by βcarotene content. Medicine, 1, 79–92. https://doi.org/10.29234/2308-9113-2021-9-1-79-92. EDN: https://elibrary.ru/JIHQOO
- Lakhanov, A. P., Kolomeichenko, V. V., & Fesenko, N. V. (2004). Morphophysiology and production process of buckwheat (435 pp.). Oryol: Oryol State Agrarian University. EDN: https://elibrary.ru/PBXPZZ
- Korobko, V. V., Pchelintseva, N. V., Luneva, M. A., & Samsonova, E. A. (2017). Features of growth and development of wheat seedlings (Triticum aestivum L.) under the influence of 2,4,6triphenyl3,5dichloropyridine and 2,6diphenyl3chloropyridine. Proceedings of Saratov University. New Series. Series: Chemistry. Biology. Ecology, 17(1), 72–78. https://doi.org/10.18500/1816-9775-2017-17-1-72-78. EDN: https://elibrary.ru/YKKJAB
- Ivanov, L. A., Ronzhina, D. A., Yudina, P. K., Zolotareva, N. V., & Kalashnikova, I. V. (2020). Seasonal dynamics of chlorophyll and carotenoid content in leaves of steppe and forest plants at species and community levels. Russian Journal of Plant Physiology, 67(3), 278–288. https://doi.org/10.31857/S0015330320030112. EDN: https://elibrary.ru/XMYDPZ
- Kurbanova, M. N., Suraeva, N. M., Rachkova, V. P., & Samoilov, A. V. (2018). Comparative study of toxic activity indicators in the Allium test. Agrarian Bulletin of the Urals, 4(171), 25–30. EDN: https://elibrary.ru/XUCOKD
- Tytereva, E. V., Ivanova, A. N., & Voitsukhovskaya, O. V. (2014). On the role of chlorophyll b in ontogenetic adaptations of plants. Komarov Botanical Institute of the RAS, 134(3), 249–256. EDN: https://elibrary.ru/SGZRUX
- Subbotin, A. M., Petrov, S. A., Malchevsky, V. A., & Khrupa, D. A. (2018). Cytogenetic analysis of the impact of Serratia fonticola bacteria isolated from permafrost samples on Allium cepa L. root system cells. In Abstracts of the XI International Conference “Plant Cell Biology in vitro and Biotechnology” (pp. 220–221). Minsk. EDN: https://elibrary.ru/YRDMFV
- Basu, S., & Tripura, K. (2021). Differential sensitivity of Allium cepa L. and Vicia faba L. to aqueous extracts of Cascabela thevetia (L.) Lippold. South African Journal of Botany, 139, 67–78. https://doi.org/10.1016/j.sajb.2021.01.033. EDN: https://elibrary.ru/UKTTBB
- Collins, T., & Margesin, R. (2019). Microbial ecology of the cryosphere (glacial and permafrost habitats): current knowledge. Applied Microbiology and Biotechnology, 103, 2537–2549. https://doi.org/10.1007/s00253-019-09631-3. EDN: https://elibrary.ru/UOMKYI
- Liang, R., Li, Z., Lau Vetter, M. C. Y., et al. (2021). Genomic reconstruction of fossil and living microorganisms in ancient Siberian permafrost. Microbiome, 9, 110. https://doi.org/10.1186/s40168-021-01057-2. EDN: https://elibrary.ru/DCXMWZ
- Renev, N. O., Rodina, E. S., Subbotin, A. M., & Malchevskiy, V. A. (2021). Influence of bacterial metabolites from permafrost on morphophysiological parameters of potato material in vitro. In International Scientific and Practical Conference “Fundamental Scientific Research and Their Applied Aspects in Biotechnology and Agriculture” (FSRAABA 2021) [BIO Web of Conferences, vol. 36, article 05009]. Tyumen. https://doi.org/10.1051/bioconf/20213605009. EDN: https://elibrary.ru/XSQJDD
- Smigocki, A. C., Heu, S., Mccanna, I., Wozniak, C., & Buta, G. (1997). Insecticidal compounds induced by regulated overproduction of cytokinins in transgenic plants. In N. Carozzi & M. Koziel (Eds.), Advances in insect control: the role of transgenic plants (pp. 225–236). Bristol.
- Jansson, J. K., & Taş, N. (2014). The microbial ecology of permafrost. Nature Reviews Microbiology, 12, 414–425. https://doi.org/10.1038/nrmicro3262. EDN: https://elibrary.ru/SOEPQH
- Bauer, M. A., Kainz, K., CarmonaGutierrez, D., & Madeo, F. (2018). Microbial wars: competition in ecological niches and within the microbiome. Microbial Cell, 5(5), 215–219.
- Panikov, N. S. (2009). Microbial activity in frozen soils. In R. Margesin (Ed.), Permafrost Soils (pp. 119–147). Innsbruck.
- Brouchkov, A. V., Trofimova, Y. B., Melnikov, V. P., Sukhovei, Y. G., Griva, G. I., Kalenova, L. F., Subbotin, A. M., Repin, V. E., Brenner, E. V., Tanaka, M., Katayama, T., & Utsumi, M. (2011). Relict microorganisms of cryolithozone as possible objects of gerontology. Advances in Gerontology, 39–44. https://doi.org/10.1134/S207905701101005X. EDN: https://elibrary.ru/RHEVRD
- Ghanem, M. E., Albacete, A., Smigocki, A. C., Frebort, I., Pospíšilová, H., MartínezAndújar, C., Acosta, M., SanchezBravo, J., Lutts, S., Dodd, I. C., & PérezAlfocea, F. (2011). Rootsynthesised cytokinins improve shoot growth and fruit yield in salinised tomato (Solanum lycopersicum L.). Journal of Experimental Botany, 62, 125–140. https://doi.org/10.1093/jxb/erq266. EDN: https://elibrary.ru/OARSPZ
- Smigocki, A. C., Heu, S., & Buta, S. G. (2000). Analysis of insecticidal activity in transgenic plants carrying the IPT plant growth hormone gene. Acta Physiologiae Plantarum, 22, 295–299. https://doi.org/10.1007/s11738-000-0038-x. EDN: https://elibrary.ru/VAHFDA
- Souza, C. P., Guedes, T. A., & Fontanetti, C. S. (2016). Evaluation of herbicides action on plant bioindicators by genetic biomarkers: a review. Environmental Monitoring and Assessment, 188.
- Kastornov, A. A., Subbotin, A. M., & Petrov, S. A. (2022). The influence of microorganisms of the Arctic paleoecosystems on the morphometric parameters and the cytogenetic apparatus of Allium cepa. In Dedicated to the 101st anniversary of the discovery of the law of homological series and the 134th anniversary of the birth of N. I. Vavilov [BIO Web of Conferences, vol. 43, article 03013]. Saratov. https://doi.org/10.1051/bioconf//20224303013. EDN: https://elibrary.ru/UCFFKT
- Timofeev, V. N., & Subbotin, A. M. (2024). Preparations based on bacteria from frozen rocks during the dressing of spring wheat seeds. BIO Web of Conferences, 108, article 10006. https://doi.org/10.1051/bioconf/202410810006. EDN: https://elibrary.ru/SMQVPA
- Wintermans, J. F., & de Mots, A. (1965). Spectrophotometric characteristics of chlorophylls a and b and their phenophytins in ethanol. Biochimica et Biophysica Acta (BBA) — Biophysics including Photosynthesis, 109, 448–453. https://doi.org/10.1016/0926-6585(65)90170-6
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