基于聚己内酯的细胞负载植入体对兔颌骨骨缺损区再生过程影响的评估

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论证。在现代再生医学中，开发用于骨组织缺损修复的个体化生物医学细胞产品具有重要意义。 此类产品通常由细胞成分、用于固定细胞并维持骨支撑功能的支架（scaffold）以及辅助成分构成。 in vitro实验无法评估该类构建体对骨组织再生过程及受体机体系统性反应的影响。

目的。确定基于聚己内酯、羟基磷灰石及牙髓干细胞的支架植入物在兔颌骨骨缺损区植入后的修复效果。

方法。研究选用苏联（俄罗斯）毛丝鼠兔（n=10），均在动物实验室条件下饲养，体重为3.5–4.5 kg，年龄为1–1.5岁。所有动物均拔除4颗牙齿（共40颗），并分为5组。于4个月后进行恢复结果的评估。为此，对植入区域进行了病理形态学研究，在苏木精–伊红染色切片中评估纤维化程度、炎症反应及骨组织重塑的表现，并通过普鲁士蓝染色分析经氧化铁纳米颗粒标记的植入细胞的分布特征。

结果。在植入区域内，支架（无论是否负载细胞）均可加速骨缺损的重塑及纤维化过程，且不形成粗大瘢痕。最佳的指标组合，即最低程度的炎症反应、骨组织重塑的最高阶段以及植入区结缔组织的成熟度，观察到于接受表面覆铜的聚己内酯支架并负载牙髓干细胞的实验组。

结论。所得结果证实，该方法在开发用于骨缺损修复的生物工程构建体方面具有良好的应用前景。

作者简介

Yuliya Dombrovskaya

North-Western State Medical University named after I.I. Mechnikov

编辑信件的主要联系方式.
Email: yuliya.dombrovskaya@szgmu.ru
ORCID iD: 0000-0001-7715-1008
SPIN 代码: 5551-8789

MD, Cand. Sci. (Medicine)

俄罗斯联邦, Saint Petersburg

Natella Enukashvily

North-Western State Medical University named after I.I. Mechnikov

Email: Natella.Enukashvili@szgmu.ru
ORCID iD: 0000-0002-5971-7917
SPIN 代码: 8161-3663

Cand. Sci. (Biology)

俄罗斯联邦, Saint Petersburg

Gleb Dubinenko

Tomsk Polytechnic University

Email: dubinenko@tpu.ru
ORCID iD: 0000-0001-9466-469X
SPIN 代码: 7129-6548
俄罗斯联邦, Tomsk

Sergei Tverdokhlebov

Tomsk Polytechnic University

Email: tverd@tpu.ru
ORCID iD: 0000-0002-2242-6358
SPIN 代码: 9005-9207

Cand. Sci. (Physics and Mathematics)

俄罗斯联邦, Tomsk

Elizaveta Rumyantseva

North-Western State Medical University named after I.I. Mechnikov

Email: lizarum2102@mail.ru
ORCID iD: 0009-0000-8118-0143
SPIN 代码: 3835-5803
俄罗斯联邦, Saint Petersburg

Arsalan Badaraev

Tomsk Polytechnic University

Email: adb6@tpu.ru
ORCID iD: 0000-0003-2800-7565
SPIN 代码: 7096-2340

Cand. Sci. (Engineering)

俄罗斯联邦, Tomsk

Varvara Bagaeva

North-Western State Medical University named after I.I. Mechnikov

Email: bagvar@mail.ru
ORCID iD: 0009-0008-5104-2872
SPIN 代码: 7510-6930
俄罗斯联邦, Saint Petersburg

Olga Kravets

North-Western State Medical University named after I.I. Mechnikov

Email: Olga.Kravetc@szgmu.ru
ORCID iD: 0009-0008-3252-0605
SPIN 代码: 4278-7900

MD, Cand. Sci. (Medicine)

俄罗斯联邦, Saint Petersburg

Anton Sakhanov

North-Western State Medical University named after I.I. Mechnikov

Email: anton.sakhanov@szgmu.ru
ORCID iD: 0000-0003-4217-6330
SPIN 代码: 8595-3308

MD, Cand. Sci. (Medicine)

俄罗斯联邦, Saint Petersburg

Olesya Dosaeva

North-Western State Medical University named after I.I. Mechnikov

Email: Olesya.dosaeva.99@mail.ru
ORCID iD: 0009-0000-6508-9518
俄罗斯联邦, Saint Petersburg

Sefiyat Bukarova

North-Western State Medical University named after I.I. Mechnikov

Email: Sofiya.bukarova@mail.ru
ORCID iD: 0009-0005-1016-9306
俄罗斯联邦, Saint Petersburg

Mikhail Kotov

North-Western State Medical University named after I.I. Mechnikov

Email: drmikhailkotov@gmail.com
ORCID iD: 0009-0000-6655-6181
SPIN 代码: 5483-9025
俄罗斯联邦, Saint Petersburg

Natalia Semenova

Almazov National Medical Research Center; Russian Research Institute of Hematology and Transfusiology Federal Medical and Biological Agency

Email: semenova@mlc-lab.ru
ORCID iD: 0000-0003-4069-0678
SPIN 代码: 3566-4723

Cand. Sci. (Biology)

俄罗斯联邦, Saint Petersburg; Saint Petersburg

Yury Novosad

H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery

Email: novosad.yur@yandex.ru
ORCID iD: 0000-0002-6150-374X
SPIN 代码: 3001-1467
俄罗斯联邦, Saint Petersburg

Platon Safonov

H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery

Email: safo165@gmail.com
ORCID iD: 0009-0006-7554-1292
SPIN 代码: 6088-1297

MD

俄罗斯联邦, Saint Petersburg

Sergei Vissarionov

H. Turner National Medical Research Center for Children’s Orthopedics and Trauma Surgery

Email: vissarionovs@gmail.com
ORCID iD: 0000-0003-4235-5048
SPIN 代码: 7125-4930

MD, Dr. Sci. (Medicine), Professor, Corresponding Member of RAS

俄罗斯联邦, Saint Petersburg

Mikhail Semenov

North-Western State Medical University named after I.I. Mechnikov

Email: mikhail.semenov@szgmu.ru
ORCID iD: 0000-0002-1295-1554
SPIN 代码: 2603-1085

MD, Dr. Sci. (Medicine), Professor

俄罗斯联邦, Saint-Petersburg

Alexey Silin

North-Western State Medical University named after I.I. Mechnikov

Email: a.silin@szgmu.ru
ORCID iD: 0000-0002-3533-5615
SPIN 代码: 4956-6941

MD, Dr. Sci. (Medicine), Professor

俄罗斯联邦, Saint Petersburg

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