Qualitative and quantitative changes of potato tuber proteome under the influence of signal molecules and infection with Phytophthora infestans

Abstract

The influence of signal molecules (jasmonic acid, phytoprostanoid oxylipins, lipopeptides of the Bacillus subtilis 26D bacterium) on the Solanum tuberosum L. potato tuber proteome was studied upon infection with the Phytophtora infestans (Mont.) de Bary phytophthorosis causative agent. Processing by signaling molecules decreased the degree of oomycete development on potato discs and caused changes in the total spectrum of soluble proteins in a pH range of 6.0–7.0 and 7.5–8.7. Differences in the differential expression of 39 polypeptides under the influence of signal molecules and infection with a pathogen were detected. It was demonstrated that the maximal protective effect upon the potato tuber infection with a pathogen is caused by the induction of protective proteins in tissues with the following values of the molecular weight/pI ratio: 24/5.8, 75/6.45, 68/6.45, 19/6.5, 69/6.3. The presence of qualitative and quantitative changes in the expression of individual proteins indicated a differential mechanism of the effect of signal molecules on the protective potential of plant cells.