Отправить статью по E-mail Achievements, Challenges, and Prospects in the Production of Nontransgenic, Genome-Edited Plants
- Авторы: Miroshnichenko D.N.1,2,3, Shulga O.A.2, Timerbaev V.R.1,2, Dolgov S.V.1,2,3
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Учреждения:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch
- All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences
- Doka-Gene Technology Ltd
- Выпуск: Том 55, № 9 (2019)
- Страницы: 825-845
- Раздел: Problems and Prospects
- URL: https://journal-vniispk.ru/0003-6838/article/view/153116
- DOI: https://doi.org/10.1134/S0003683819090047
- ID: 153116
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Аннотация
The review summarizes the problems, achievements, and prospects of various approaches related to the delivery of components of DNA editing systems to plant cells, the regeneration of whole plants with an edited genome, and the development of transgene-free or transgene-clean crops. Here, an attempt was made to systematize the results of various studies describing the successful production of genome-edited (GE) plants with various genome modifications/mutations via the application of nuclease DNA–editing systems (ZFN, TALEN, and CRISPR/Cas). We discuss the main directions for the development of nuclease-based genome-editing methods to obtain GE plants free of foreign sequences from genome-editing tools.
Об авторах
D. Miroshnichenko
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch; All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences; Doka-Gene Technology Ltd
Email: dolgov@bibch.ru
Россия, Pushchino, Moscow oblast, 142290; Moscow, 127550; Rogachevo, Moscow oblast, 141880
O. Shulga
All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences
Email: dolgov@bibch.ru
Россия, Moscow, 127550
V. Timerbaev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch; All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences
Email: dolgov@bibch.ru
Россия, Pushchino, Moscow oblast, 142290; Moscow, 127550
S. Dolgov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino Branch; All-Russia Institute of Agricultural Biotechnology, Russian Academy of Sciences; Doka-Gene Technology Ltd
Автор, ответственный за переписку.
Email: dolgov@bibch.ru
Россия, Pushchino, Moscow oblast, 142290; Moscow, 127550; Rogachevo, Moscow oblast, 141880
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