Spreading of Tissue Spheroids on an Electrospun Polyurethane Matrix

Abstract

Tissue spheroids formed from fibroblasts using a micromolded non-adhesive hydrogel were located using a three-dimensional (3D) bioprinter on the surface of a nanofibrous polyurethane matrix produced by electrospinning. It was shown that the tissue spheroids attach to the matrix surface within a few hours and completely flatten after several days, indicating high biocompatibility of the matrix used. Tissue structures formed by the attachment and spreading of tissue spheroids on an electrospun matrix are a new technological platform for biofabrication and 3D bioprinting of tissues and organs.