Email this article New Silk Fibroin-Based Bioresorbable Microcarriers
- Authors: Arkhipova A.Y.1, Kotlyarova M.C.1, Novichkova S.G.1, Agapova O.I.2, Kulikov D.A.3, Kulikov A.V.4, Drutskaya M.S.5, Agapov I.I.2, Moisenovich M.M.1
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Affiliations:
- Biological Faculty, M. V. Lomonosov Moscow State University
- V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
- M. F. Vladimirsky Moscow Regional Research and Clinical Institute
- Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
- V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- Issue: Vol 160, No 4 (2016)
- Pages: 491-494
- Section: Biotechnologies
- URL: https://journal-vniispk.ru/0007-4888/article/view/236752
- DOI: https://doi.org/10.1007/s10517-016-3204-x
- ID: 236752
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Abstract
We fabricated bioresorbable microcarriers from water solution of Bombyx mori silk fi broin. The microcarriers are 3D structures with intricate surface and pores allowing penetration of culture medium, gas exchange, and cell adhesion. Fibroin molecules form hydrophobic structures and normally have a negative charge, which stimulates migration, but inhibits cell adhesion and makes it less effective. In order to improve adhesion efficiency and velocity, gelatin (hydrophilic biopolymer with integrin-recognizing RGD sequence) was added to the microcarrier composition. The resultant bioresorbable microcarriers support adhesion and proliferation of 3T3 murine fibroblasts.
About the authors
A. Yu. Arkhipova
Biological Faculty, M. V. Lomonosov Moscow State University
Email: igor_agapov@mail.ru
Russian Federation, Moscow
M. C. Kotlyarova
Biological Faculty, M. V. Lomonosov Moscow State University
Email: igor_agapov@mail.ru
Russian Federation, Moscow
S. G. Novichkova
Biological Faculty, M. V. Lomonosov Moscow State University
Email: igor_agapov@mail.ru
Russian Federation, Moscow
O. I. Agapova
V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
Email: igor_agapov@mail.ru
Russian Federation, Moscow
D. A. Kulikov
M. F. Vladimirsky Moscow Regional Research and Clinical Institute
Email: igor_agapov@mail.ru
Russian Federation, Moscow
A. V. Kulikov
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
Email: igor_agapov@mail.ru
Russian Federation, Moscow
M. S. Drutskaya
V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: igor_agapov@mail.ru
Russian Federation, Moscow
I. I. Agapov
V. I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation
Author for correspondence.
Email: igor_agapov@mail.ru
Russian Federation, Moscow
M. M. Moisenovich
Biological Faculty, M. V. Lomonosov Moscow State University
Email: igor_agapov@mail.ru
Russian Federation, Moscow
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