Email this article Morphofunctional Characteristics of Human Lymphocytes after In Vitro Activation
- Authors: Abakushina E.V.1, Marizina Y.V.1, Kaprin A.D.1
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Affiliations:
- A. Tsyb Medical Radiological Research Centre – Branch of the National Medical Research Radiological Centre, the Ministry of Health of the Russian Federation
- Issue: Vol 161, No 5 (2016)
- Pages: 731-735
- Section: Article
- URL: https://journal-vniispk.ru/0007-4888/article/view/237725
- DOI: https://doi.org/10.1007/s10517-016-3496-x
- ID: 237725
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Abstract
We describe a method of activation of lymphocytes isolated from the peripheral blood of patients with cutaneous melanoma and cultured in serum-free medium in the presence of IL-2 and IL-15. Viability, proliferative, cytotoxic, and functional activities of lymphocytes are evaluated. The lymphocytes actively proliferated in this nutrient medium and can be activated in vitro. The method for obtaining sufficient amount of activated lymphocytes can be recommended for adoptive cell immunotherapy of cancer patients.
About the authors
E. V. Abakushina
A. Tsyb Medical Radiological Research Centre – Branch of the National Medical Research Radiological Centre, the Ministry of Health of the Russian Federation
Author for correspondence.
Email: abakushina@mail.ru
Russian Federation, Obninsk
Yu. V. Marizina
A. Tsyb Medical Radiological Research Centre – Branch of the National Medical Research Radiological Centre, the Ministry of Health of the Russian Federation
Email: abakushina@mail.ru
Russian Federation, Obninsk
A. D. Kaprin
A. Tsyb Medical Radiological Research Centre – Branch of the National Medical Research Radiological Centre, the Ministry of Health of the Russian Federation
Email: abakushina@mail.ru
Russian Federation, Obninsk
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