Test System for Amplification of the Complete Mitochondrial Genome of Siberian Sturgeon (Acipenser baerii)

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Abstract

A PCR system for amplification of the complete mitochondrial genome of the Siberian sturgeon (Acipenser baerii) has been developed, allowing to obtain six overlapping amplicons for sequencing by the NGS method. DNA sequencing of 24 representatives of aquacultured Siberian sturgeon of the Lena origin was carried out. The data on the extremely low diversity of mtDNA in the Lena sturgeon bred in aquaculture conditions have been confirmed. Heteroplasmic regions have been detected in all sequenced samples.

About the authors

N. V Bardukov

Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst

Dubrovitsy, Moscow Region, Russia

N. B Pisarenko

Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst

Dubrovitsy, Moscow Region, Russia

A. K Nikipelova

Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst

Dubrovitsy, Moscow Region, Russia

V. I Nikipelov

Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst

Dubrovitsy, Moscow Region, Russia

A. N Rodionov

Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst

Dubrovitsy, Moscow Region, Russia

A. V Dotsev

Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst

Dubrovitsy, Moscow Region, Russia

M. E Romazeva

Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst

Dubrovitsy, Moscow Region, Russia

A. A Belous

Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst; All-Russian Research Institute of Integral Fish Farming – Branch of the Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst

Email: belousa663@gmail.com
Dubrovitsy, Moscow Region, Russia

P. I Otradnov

Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst

Dubrovitsy, Moscow Region, Russia

N. A Zinovieva

Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst

Dubrovitsy, Moscow Region, Russia

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