Email this article Application of deuterium–hydrogen exchange to study the secondary structure of oligonucleotide ions in a gas phase
- Authors: Kostyukevich Y.I.1,2,3, Kononikhin A.S.2,3,4, Popov I.A.5,3, Bugrova A.E.5,4, Starodubtseva N.L.3,4, Nikolaev E.N.1,2,3
-
Affiliations:
- Skolkovo Institute of Science and Technology
- Talrose Institute of Energy Problems of Chemical Physics
- Moscow Institute of Physics and Technology (State University)
- Kulakov Research Center for Obstetrics, Gynecology, and Perinatology
- Emanuel Institute of Biochemical Physics
- Issue: Vol 50, No 6 (2016)
- Pages: 427-432
- Section: General Aspects of High Energy Chemistry
- URL: https://journal-vniispk.ru/0018-1439/article/view/157066
- DOI: https://doi.org/10.1134/S0018143916060096
- ID: 157066
Cite item
Open Access
Access granted
Subscription Access
Abstract
At present, the secondary structure of oligonucleotide ions in a gas phase is almost not understood. One of the main points is the retention of a hairpin secondary structure during ionization. In this work, we used a deuterium–hydrogen exchange reaction in a gas phase at atmospheric pressure for studying the conformational dynamics of oligonucleotide ions formed as a result of electrospray ionization. The exchange reactions of two oligonucleotides, which consisted of the identical sets of nucleotides but differed in their sequence, have been studied. One of these oligonucleotides formed a hairpin secondary structure, but the other did not. It has been found that both of the oligonucleotides demonstrate similar reaction dynamics of deuterium/hydrogen exchange in the gas phase; thereby indicating that the secondary structure has been completely destroyed during ionization.
About the authors
Yu. I. Kostyukevich
Skolkovo Institute of Science and Technology; Talrose Institute of Energy Problems of Chemical Physics; Moscow Institute of Physics and Technology (State University)
Author for correspondence.
Email: yura542@gmail.com
Russian Federation, Skolkovo, Moscow oblast, 143025; Moscow, 119334; Dolgoprudnyi, Moscow oblast, 141700
A. S. Kononikhin
Talrose Institute of Energy Problems of Chemical Physics; Moscow Institute of Physics and Technology (State University); Kulakov Research Center for Obstetrics, Gynecology, and Perinatology
Email: yura542@gmail.com
Russian Federation, Moscow, 119334; Dolgoprudnyi, Moscow oblast, 141700; Moscow, 117997
I. A. Popov
Emanuel Institute of Biochemical Physics; Moscow Institute of Physics and Technology (State University)
Email: yura542@gmail.com
Russian Federation, Moscow, 119334; Dolgoprudnyi, Moscow oblast, 141700
A. E. Bugrova
Emanuel Institute of Biochemical Physics; Kulakov Research Center for Obstetrics, Gynecology, and Perinatology
Email: yura542@gmail.com
Russian Federation, Moscow, 119334; Moscow, 117997
N. L. Starodubtseva
Moscow Institute of Physics and Technology (State University); Kulakov Research Center for Obstetrics, Gynecology, and Perinatology
Email: yura542@gmail.com
Russian Federation, Dolgoprudnyi, Moscow oblast, 141700; Moscow, 117997
E. N. Nikolaev
Skolkovo Institute of Science and Technology; Talrose Institute of Energy Problems of Chemical Physics; Moscow Institute of Physics and Technology (State University)
Email: yura542@gmail.com
Russian Federation, Skolkovo, Moscow oblast, 143025; Moscow, 119334; Dolgoprudnyi, Moscow oblast, 141700
Supplementary files
