Email this article Femtosecond absorption spectroscopy of cytochrome c oxidase: Excited electronic states and relaxation processes in heme a and heme a3 centers
- Authors: Shelaev I.V.1, Gostev F.E.1, Vygodina T.V.2, Lepeshkevich S.V.3, Dzhagarov B.M.3
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Affiliations:
- Semenov Institute of Chemical Physics
- Belozerski Institute of Physicochemical Biology
- Stepanov Institute of Physics
- Issue: Vol 52, No 1 (2018)
- Pages: 45-51
- Section: Photonics
- URL: https://journal-vniispk.ru/0018-1439/article/view/157318
- DOI: https://doi.org/10.1134/S0018143918010101
- ID: 157318
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Abstract
Cytochrome c oxidase, the key bioenergetic protein, was studied by femtosecond absorption spectroscopy. Time-resolved spectral characteristics of the difference spectra recorded in the timescale from 80 fs to 20 ps were analyzed. Electronic relaxation of the excitation energy in heme a occurs in three successive steps. After completion of these steps, heme a is in the excited vibrational state of the ground state. Vibrational relaxation, cooling of the heme, occurs for several picoseconds.
About the authors
I. V. Shelaev
Semenov Institute of Chemical Physics
Email: b.dzhagarov@ifanbel.bas-net.by
Russian Federation, Moscow, 119991
F. E. Gostev
Semenov Institute of Chemical Physics
Email: b.dzhagarov@ifanbel.bas-net.by
Russian Federation, Moscow, 119991
T. V. Vygodina
Belozerski Institute of Physicochemical Biology
Email: b.dzhagarov@ifanbel.bas-net.by
Russian Federation, Moscow, 119991
S. V. Lepeshkevich
Stepanov Institute of Physics
Email: b.dzhagarov@ifanbel.bas-net.by
Belarus, Minsk, 220072
B. M. Dzhagarov
Stepanov Institute of Physics
Author for correspondence.
Email: b.dzhagarov@ifanbel.bas-net.by
Belarus, Minsk, 220072
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