Decomposition of Small-Angle Scattering Profiles from Two Conformational States of 3-Isopropylmalate Dehydrogenase Using Evolving Factor Analysis

P. V. Konarev; Конарев П. В.; V. V. Volkov; Волков В. В.

doi:10.31857/S0023476123600155

Decomposition of Small-Angle Scattering Profiles from Two Conformational States of 3-Isopropylmalate Dehydrogenase Using Evolving Factor Analysis

Autores: Konarev P.V.¹^,2, Volkov V.V.¹^,3
Afiliações:
1. Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia
2. National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia
3. National Research Centre “Kurchatov Institute,”, 123182, Moscow, Russia
Edição: Volume 68, Nº 6 (2023)
Páginas: 946-950
Seção: ДИФРАКЦИЯ И РАССЕЯНИЕ ИОНИЗИРУЮЩИХ ИЗЛУЧЕНИЙ
URL: https://journal-vniispk.ru/0023-4761/article/view/231843
DOI: https://doi.org/10.31857/S0023476123600155
EDN: https://elibrary.ru/XRTPYF
ID: 231843

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Resumo

The separation of two conformational states of 3-isopropylmalate dehydrogenase molecules from Thermus thermophilus in solution on a gel chromatographic column, attached to a sample cell of a small-angle X-ray scattering synchrotron beamline, has been simulated. The scattering intensity profiles from the open and closed forms of the enzyme molecules were restored by evolving factor analysis (EFA) using the synthetic data set with added Poisson noise at the relative level of 3–5%. Thus, the efficiency of the EFA algorithm is confirmed in the case of two-component mixtures consisting of particles with the same molecular masses.

Sobre autores

P. Konarev

Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia; National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia

Email: peter_konarev@mail.ru
Россия, Москва; Россия, Москва

V. Volkov

Autor responsável pela correspondência
Email: vvo@crys.ras.ru
Россия, Москва; Россия, Москва

Bibliografia

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