Revised Sequence and Annotation of Burkholderia pseudomallei/thailandensis Bacteriophage Bp-AMP1—A Potential Agent of Natural Biocontrol of the Populations of the Melioidosis Causative Agent

Abstract

Significant impact of Bp-AMP1-related bacteriophages on the population dynamics of Burkholderia pseudomallei in agroecosystems of the regions endemic for melioidosis has been repeatedly hypothesised in recent years. Temperature-dependent life cycle of these viruses may be responsible for modulation of the natural phage-mediated biocontrol, and thus it may contribute to the complexity of the seasonal dynamics of melioidosis observed. Refined data on Bp-AMP1 phage genome organization are a prerequisite for the ongoing research on infection physiology of this virus. In the present work, location of the physical ends of the virion-encapsidated phage DNA was determined, 389-bp long terminal repeats were revealed, and the annotation of the phage Bp-AMP1 genome sequence was revised.