Methylomonas Montana  sp. nov., the First Unpigmented Methanotroph of the Genus  Methylomonas , Isolated from Mountain River Sediments

R. Z. Suleimanov; Сулейманов Р. З.; E. N. Tikhonova; Тихонова Е. Н.; I. Y. Oshkin; Ошкин И. Ю.; O. V. Danilova; Данилова О. В.; S. N. Dedysh; Дедыш С. Н.

doi:10.31857/S0026365623600426

Methylomonas Montana sp. nov., the First Unpigmented Methanotroph of the Genus Methylomonas, Isolated from Mountain River Sediments

Authors: Suleimanov R.Z.¹, Tikhonova E.N.¹, Oshkin I.Y.¹, Danilova O.V.¹, Dedysh S.N.¹
Affiliations:
1. Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences
Issue: Vol 92, No 6 (2023)
Pages: 535-544
Section: EXPERIMENTAL ARTICLES
URL: https://journal-vniispk.ru/0026-3656/article/view/231882
DOI: https://doi.org/10.31857/S0026365623600426
EDN: https://elibrary.ru/BPCTBQ
ID: 231882

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Abstract

Abstract—

Aerobic methanotrophic bacteria of the genus Methylomonas inhabit a wide spectrum of habitats including freshwater bodies, river sediments, wetlands, rice paddies, landfill cover soils, and hydromorphic soils. Majority of the currently described species of this genus are represented by neutrophilic, motile, growing on methane rod-shaped bacteria, whose pigmentation varies from yellow to pink and red. This study reports characterization of a novel, unpigmented isolate of these bacteria, strain MW1^T, which was obtained from sediments of the mountain river Khosta, Krasnodar region, Russia. Strain MW1^T grew on methane and methanol within a temperature range of 8–37°C (optimum at 25–30°C) and at рН 5.5–7.5 (optimum at 6.3–7.0). The 16S rRNA gene sequence of strain MW1^T displayed 95.48–98.47% similarity to those in earlier described Methylomonas. The closest taxonomically characterized phylogenetic relative of strain MW1^T was M. fluvii EbB^T, isolated from the river Elbe sediments. Complete genome sequence of strain MW1^T was 4.6 Mb in size and contained three rRNA operons and about 4200 protein-encoding genes, including the gene cluster pmoCAB coding for membrane methane monooxygenase. Soluble methane monooxygenase was not encoded in the genome. The G+C DNA content was 52.4%. The average nucleotide identity of the genome of strain MW1^T with those in earlier described representatives of the genus Methylomonas was 79.4–82.1%. We propose to classify this isolate as representing a novel species of the genus Methylomonas, M. montana sp. nov. Strain MW1^T (=VKM 3737^T = UQM 41536^T) is the type strain of the newly proposed species.

Keywords

methanotrophic bacteria, genus Methylomonas, Methylomonas montana, growth on methane, freshwater sediments, genome analysis

References

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