Email this article Regulation of m6A RNA Methylation and Its Effect on Myogenic Differentiation in Murine Myoblasts
- Authors: Chen J.N.1, Chen Y.1, Wei Y.Y.1, Raza M.A.2, Zou Q.1, Xi X.Y.1, Zhu L.3, Tang G.Q.3, Jiang Y.Z.1, Li X.W.3
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Affiliations:
- Department of Zoology, College of Life Science, Sichuan Agricultural University
- Department of Crop Cultivation and Farming System, College of Agronomy, Sichuan Agricultural University
- Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University
- Issue: Vol 53, No 3 (2019)
- Pages: 384-392
- Section: Molecular Cell Biology
- URL: https://journal-vniispk.ru/0026-8933/article/view/163945
- DOI: https://doi.org/10.1134/S002689331903004X
- ID: 163945
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Abstract
N6-methyladenosine (m6A) has been identified as a conserved epitranscriptomic modification of eukaryotic mRNAs, and plays important biological roles in the regulation of cellular metabolic processes. However, its role in myogenic differentiation is unclear. Here, we altered the m6A RNA methylation level by overexpression of METTL3, and explored the effect of m6A RNA methylation on myogenic differentiation of murine myoblasts in vitro. The m6A RNA methylation level is regulated by exogenous methylation inhibitor cycloleucine (Cyc) and methyl donor betaine (Bet). Therefore, chemical reagents of Cyc and Bet were used to test the regulatory effect of m6A RNA methylation on myogenic differentiation. Results showed that METTL3 and Bet positively regulated the m6A RNA methylation levels, and Cyc negatively regulated m6A RNA methylation levels. In addition, m6A methylation positively regulated myogenic differentiation in murine myoblasts. These findings provide insight in the mechanisms underlying the effect of m6A RNA methylation on myogenesis.
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About the authors
J. N. Chen
Department of Zoology, College of Life Science, Sichuan Agricultural University
Email: xuewei.li@sicau.edu.cn
China, Ya’an, Sichuan, 625014
Y. Chen
Department of Zoology, College of Life Science, Sichuan Agricultural University
Email: xuewei.li@sicau.edu.cn
China, Ya’an, Sichuan, 625014
Y. Y. Wei
Department of Zoology, College of Life Science, Sichuan Agricultural University
Email: xuewei.li@sicau.edu.cn
China, Ya’an, Sichuan, 625014
M. A. Raza
Department of Crop Cultivation and Farming System, College of Agronomy, Sichuan Agricultural University
Email: xuewei.li@sicau.edu.cn
China, Chengdu, Sichuan, 611130
Q. Zou
Department of Zoology, College of Life Science, Sichuan Agricultural University
Email: xuewei.li@sicau.edu.cn
China, Ya’an, Sichuan, 625014
X. Y. Xi
Department of Zoology, College of Life Science, Sichuan Agricultural University
Email: xuewei.li@sicau.edu.cn
China, Ya’an, Sichuan, 625014
L. Zhu
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University
Email: xuewei.li@sicau.edu.cn
China, Chengdu, Sichuan, 611130
G. Q. Tang
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University
Email: xuewei.li@sicau.edu.cn
China, Chengdu, Sichuan, 611130
Y. Z. Jiang
Department of Zoology, College of Life Science, Sichuan Agricultural University
Author for correspondence.
Email: jiangyz04@163.com
China, Ya’an, Sichuan, 625014
X. W. Li
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University
Author for correspondence.
Email: xuewei.li@sicau.edu.cn
China, Chengdu, Sichuan, 611130
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