MiR-222-3p Regulates the Proliferation and Differentiation of C2C12 Myoblasts by Targeting BTG2

D. L. Yang; M. L. Gan; Y. Tan; G. H. Ge; Q. Li; Y. Z. Jiang; G. Q. Tang; M. Z. Li; J. Y. Wang; X. W. Li; S. H. Zhang; L. Zhu

doi:10.1134/S0026893319010187

MiR-222-3p Regulates the Proliferation and Differentiation of C2C12 Myoblasts by Targeting BTG2

Авторлар: Yang D.L.¹, Gan M.L.¹, Tan Y.¹, Ge G.H.¹, Li Q.², Jiang Y.Z.³, Tang G.Q.¹, Li M.Z.¹, Wang J.Y.⁴, Li X.W.¹, Zhang S.H.¹, Zhu L.¹
Мекемелер:
1. Farm Animal Genetics Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University
2. Sichuan Province General Station of Animal Husbandry
3. College of Life and Science, Sichuan Agricultural University
4. Chongqing Academy of Animal Sciences
Шығарылым: Том 53, № 1 (2019)
Беттер: 38-44
Бөлім: Molecular Cell Biology
URL: https://journal-vniispk.ru/0026-8933/article/view/163787
DOI: https://doi.org/10.1134/S0026893319010187
ID: 163787

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат
Рұқсат жабық

Рұқсат берілді
Рұқсат жабық

Тек жазылушылар үшін

Аннотация
Авторлар туралы
Әдебиет тізімі
Қосымша файлдар
Статистика

Аннотация

MiR-222-3p has been implicated in tumor cell proliferation and has an important role in the differentiation and maturation of myogenic cells. However, its role in skeletal myoblast proliferation is still unclear. In this study, we found that miR-222-3p expression increases initially and then decreases during C2C12 myoblast proliferation. Using synthetic miRNA mimics and inhibitors in gain- or loss-of-function experiments, we showed that miR-222-3p overexpression in C2C12 cells promotes myoblast proliferation and represses myofiber formation, while miR-222-3p downregulation has the opposite effect. Using a prediction program, BTG2 was identified as a possible target gene of miR-222-3p. During myogenesis, miR-222-3p mimics repress BTG2 expression, while miR-222-3p inhibitors promote BTG2 expression. Using dual-luciferase reporter assay, we further demonstrated that miR-222-3p specifically targets BTG2. Additionally, we show that siRNA-mediated downregulation of BTG2 expression in C2C12 myoblasts promotes the proliferation and suppresses differentiation. In conclusion, we provide a novel insight into the mechanism by which miR-222-3p regulates the proliferation and differentiation of C2C12 myoblasts by targeting BTG2. This information contributes to our understanding of the role of miRNAs in skeletal muscle development.

Негізгі сөздер

MiR-222-3p, BTG2, C2C12 myoblasts, proliferation, differentiation

Қосымша файлдар

Әрекет

1. JATS XML

Жүктеу

Пайдаланушының аты
Құпиясөз
Мені есте сақтау

Құпия сөзді ұмыттыңыз ба?	Тіркеу

Пайдаланушының аты
Құпиясөз
Мені есте сақтау

Құпия сөзді ұмыттыңыз ба?	Тіркеу

MiR-222-3p Regulates the Proliferation and Differentiation of C2C12 Myoblasts by Targeting BTG2

Толық мәтін

Аннотация

Негізгі сөздер

Авторлар туралы

D. Yang

M. Gan

Y. Tan

G. Ge

Q. Li

Y. Jiang

G. Tang

M. Li

J. Wang

X. Li

S. Zhang

L. Zhu

Қосымша файлдар