Enviar artigo por via de e-mail Overexpression of microRNAs miR-9, -98, and -199 Correlates with the Downregulation of HK2 Expression in Colorectal Cancer
- Autores: Snezhkina A.V.1, Krasnov G.S.1, Zhikrivetskaya S.O.1, Karpova I.Y.1, Fedorova M.S.1, Nyushko K.M.2, Belyakov M.M.2, Gnuchev N.V.3, Sidorov D.V.2, Alekseev B.Y.2, Melnikova N.V.1, Kudryavtseva A.V.1,2
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Afiliações:
- Engelhardt Institute of Molecular Biology
- National Medical Research Radiological Center
- Institute of Gene Biology
- Edição: Volume 52, Nº 2 (2018)
- Páginas: 190-199
- Seção: Genomics. Transcriptomics
- URL: https://journal-vniispk.ru/0026-8933/article/view/163454
- DOI: https://doi.org/10.1134/S0026893318020140
- ID: 163454
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Resumo
Glycolysis activation is one of the main features of energy metabolism in cancer cells that is associated with the increase in glycolytic enzyme synthesis, primarily, hexokinases (HKs), in many types of tumors. Conversely, in colorectal cancer (CRC) the decrease in the expression of HK2 gene, which encodes one of the key rate-limiting enzyme of glycolysis, was revealed, thus, the study of the mechanisms of its inhibition in CRC is of particular interest. To search for potential microRNAs, inhibiting the expression of HK2 in CRC, we have performed the analysis of data from “The Cancer Genome Atlas” (TCGA) and five microRNA–mRNA target interaction databases (TargetScan, DIANA microT, mirSVR (miRanda), PicTar, and miRTarBase) using original CrossHub software. Seven microRNAs containing binding site on mRNA HK2, which expression is negatively correlated with HK2 expression, were selected for further analysis. The expression levels of these microRNAs and mRNA HK2 were estimated by quantitative PCR on a set of CRC samples. It has been shown, that the expression of three microRNAs (miR-9-5p, -98-5p, and -199-5p) was increased and correlated negatively with mRNA level of HK2 gene. Thus, downregulation of HK2 gene may be caused by its negative regulation through microRNAs miR-9-5p, -98-5p, and -199-5p.
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Sobre autores
A. Snezhkina
Engelhardt Institute of Molecular Biology
Email: rhizamoeba@mail.ru
Rússia, Moscow, 119991
G. Krasnov
Engelhardt Institute of Molecular Biology
Email: rhizamoeba@mail.ru
Rússia, Moscow, 119991
S. Zhikrivetskaya
Engelhardt Institute of Molecular Biology
Email: rhizamoeba@mail.ru
Rússia, Moscow, 119991
I. Karpova
Engelhardt Institute of Molecular Biology
Email: rhizamoeba@mail.ru
Rússia, Moscow, 119991
M. Fedorova
Engelhardt Institute of Molecular Biology
Email: rhizamoeba@mail.ru
Rússia, Moscow, 119991
K. Nyushko
National Medical Research Radiological Center
Email: rhizamoeba@mail.ru
Rússia, Moscow, 125284
M. Belyakov
National Medical Research Radiological Center
Email: rhizamoeba@mail.ru
Rússia, Moscow, 125284
N. Gnuchev
Institute of Gene Biology
Email: rhizamoeba@mail.ru
Rússia, Moscow, 119334
D. Sidorov
National Medical Research Radiological Center
Email: rhizamoeba@mail.ru
Rússia, Moscow, 125284
B. Alekseev
National Medical Research Radiological Center
Email: rhizamoeba@mail.ru
Rússia, Moscow, 125284
N. Melnikova
Engelhardt Institute of Molecular Biology
Email: rhizamoeba@mail.ru
Rússia, Moscow, 119991
A. Kudryavtseva
Engelhardt Institute of Molecular Biology; National Medical Research Radiological Center
Autor responsável pela correspondência
Email: rhizamoeba@mail.ru
Rússia, Moscow, 119991; Moscow, 125284
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