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Anti-Restriction Activity of ArdB Protein against EcoAI Endonuclease
- Authors: Kudryavtseva A.A.1, Alekhin V.A.2, Lebedeva M.D.2, Csefalvay E.3, Weiserova .4, Manukhov I.V.1,5
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Affiliations:
- Moscow Institute of Physics and Technology
- “Biophystech” LLC
- Laboratory of Structural Biology and Bioinformatics, Institute of Microbiology, Academy of Sciences of the Czech Republic
- Institute of Microbiology, Academy of Sciences of the Czech Republic
- Moscow State University of Food Production
- Issue: Vol 57, No 1 (2023)
- Pages: 101-105
- Section: МОЛЕКУЛЯРНАЯ БИОЛОГИЯ КЛЕТКИ
- URL: https://journal-vniispk.ru/0026-8984/article/view/138616
- DOI: https://doi.org/10.31857/S0026898423010056
- EDN: https://elibrary.ru/AWVMVU
- ID: 138616
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Abstract
ArdB proteins are known to inhibit the activity of type I restriction–modification (RM-I) system, in particular EcoKI (family IA). The mechanism of ArdB’s activity still remains unknown; the spectrum of targets inhibited by them has been poorly studied. In this work, it was shown that the presence of the ardB gene from R64 plasmid could suppress the activity of EcoAI endonuclease (IB family) in Escherichia coli TG1 cells. The absence of specificity of ArdB to a certain RM-I system (it inhibits both the IA- and IB-family), it can be a-ssumed that the mechanism of the anti-restriction activity of this protein does not depend on both the s-equence DNA at the recognition site and the structure of the restrictase of the RM-I systems.
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About the authors
A. A. Kudryavtseva
Moscow Institute of Physics and Technology
Author for correspondence.
Email: kudryavtseva@phystech.edu
Russia, 141707, Moscow Region, Dolgoprudny
V. A. Alekhin
“Biophystech” LLC
Email: kudryavtseva@phystech.edu
Russia, 141701, Moscow Region, Dolgoprudny
M. D. Lebedeva
“Biophystech” LLC
Email: kudryavtseva@phystech.edu
Russia, 141701, Moscow Region, Dolgoprudny
E. Csefalvay
Laboratory of Structural Biology and Bioinformatics, Institute of Microbiology, Academy of Sciences of the Czech Republic
Email: kudryavtseva@phystech.edu
Czech Republic, 37333 , Nove Hrady, Zamek 136
M. Weiserova
Institute of Microbiology, Academy of Sciences of the Czech Republic
Email: kudryavtseva@phystech.edu
Czech Republic, 14220, Praha 4,, Vídeňská 1083
I. V. Manukhov
Moscow Institute of Physics and Technology; Moscow State University of Food Production
Email: kudryavtseva@phystech.edu
Russia, 141707, Moscow Region, Dolgoprudny; Russia, 125080, Moscow
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