Latex Agglutination as an Alternative to Hemagglutination Reaction of Influenza Viruses

P. A. Ivanov; Иванов П. А.; A. V. Lyashko; Ляшко А. В.; S. A. Ionov; Ионов С. А.; D. N. Shcherbinin; Щербинин Д. В.; I. A. Rudneva; Руднева И. А.; A. A. Shilov; Шилов А. А.; N. I. Bunkova; Бунькова Н. И.; M. M. Shmarov; Шмаров М. М.; T. A. Timofeeva; Тимофеева Т. А.

doi:10.31857/S0026898423040092

Latex Agglutination as an Alternative to Hemagglutination Reaction of Influenza Viruses

Authors: Ivanov P.A.¹^,2, Lyashko A.V.¹, Ionov S.A.¹^,3, Shcherbinin D.N.¹, Rudneva I.A.¹, Shilov A.A.¹, Bunkova N.I.¹, Shmarov M.M.¹^,4, Timofeeva T.A.¹
Affiliations:
1. National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya, Ministry of Health of the Russian Federation
2. Winogradsky Institute of Microbiology RC Biotechnology, Russian Academy of Sciences
3. Mendeleev University of Chemical Technology
4. Sechenov First Moscow State University, Ministry of Health of the Russian Federation
Issue: Vol 57, No 5 (2023)
Pages: 898-906
Section: МЕТОДЫ
URL: https://journal-vniispk.ru/0026-8984/article/view/138713
DOI: https://doi.org/10.31857/S0026898423040092
EDN: https://elibrary.ru/QLOTLJ
ID: 138713

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Abstract

As an alternative to the classical method of erythrocyte hemagglutination, a latex agglutination assay based on the interaction of influenza viruses with the sialoglycoprotein fetuin immobilized on the surface of polystyrene microspheres, has been developed. Twelve influenza A virus strains of different subtypes and two influenza B viruses of different lines were tested. Simultaneous titration of viruses using the classical hemagglutination test and the proposed latex agglutination assay showed similar sensitivity and a high degree of correlation (R = 0.94). The obtained microspheres can be used for titration of viruses that recognize and bind sialylated glycans as receptors. In particular, latex aggregation was also induced by the Newcastle disease virus.

Keywords

influenza viruses, microspheres, fetuin, latex agglutination reaction, hemagglutin, receptor binding specificity

References

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