Email this article Obtaining DNA aptamers to human interleukin-6 for biomagnetic immunoassay nanosensors
- Authors: Spiridonova V.A.1, Novikova T.M.1, Snigirev O.V.2
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Affiliations:
- Belozersky Institute of Physicochemical Biology
- Department of Physics
- Issue: Vol 71, No 1 (2016)
- Pages: 135-138
- Section: Biophysics and Medical Physics
- URL: https://journal-vniispk.ru/0027-1349/article/view/164435
- DOI: https://doi.org/10.3103/S002713491601015X
- ID: 164435
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Abstract
We used aptamers, which are functional equivalents of antibodies, in order to develop a nanosensor immunoassay system based on magnetic nanoparticles and a SQUID magnetometer. Selection was used to obtain DNA aptamers to interleukin-6; their affinity to the target protein was characterized by surface plasmon resonance. It was shown that the biotinylated aptamer binds to magnetic nanoparticles that were functionalized with streptavidin.
About the authors
V. A. Spiridonova
Belozersky Institute of Physicochemical Biology
Email: osnig@inbox.ru
Russian Federation, Moscow, 119991
T. M. Novikova
Belozersky Institute of Physicochemical Biology
Email: osnig@inbox.ru
Russian Federation, Moscow, 119991
O. V. Snigirev
Department of Physics
Author for correspondence.
Email: osnig@inbox.ru
Russian Federation, Moscow, 119991
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