The effect of plasmon silver and exiton semiconductor nanoparticles on the bacteriorhodopsin photocycle in Halobacterium salinarum membranes
- Авторы: Oleinikov V.A.1,2, Lukashev E.P.3, Zaitsev S.Y.4, Chistyakov A.A.1,2, Solovyeva D.O.1,2, Mochalov K.E.1,2, Nabiev I.1,5
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Учреждения:
- National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Moscow State University
- Skryabin State Academy of Veterinary Medicine and Biotechnology
- University of Reims Champagne-Ardenne
- Выпуск: Том 122, № 1 (2017)
- Страницы: 30-35
- Раздел: International Conference “Photonic Colloidal Nanostructures: Synthesis, Properties, and Applications” (PCNSPA-2016)
- URL: https://journal-vniispk.ru/0030-400X/article/view/165291
- DOI: https://doi.org/10.1134/S0030400X17010210
- ID: 165291
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Аннотация
The interaction of semiconductor quantum dots and silver nanoparticles (AgNPs) with bacteriorhodopsin (BR), a membrane protein contained in the purple membrane (PM) of Halobacterium salinarum, is studied. It is shown that both types of nanoparticles are adsorbed efficiently on the surface of the purple membranes, modulating the parameters of the bacteriorhodopsin photocycle. Electrostatic interactions are found to be the main cause of the effect of nanoparticles on the bacteriorhodopsin photocycle. These results explain our earlier data on the “fixation” of the bacteriorhodopsin photocycle for protein molecules trapped after incubation of the purple membranes with silver nanoparticles near the location of the “hot spots” of the effect of surface-enhanced Raman scattering (SERS). It is demonstrated that exposure of silver nanoparticles with bacteriorhodopsin in SERS-active regions lowers the amount of bacteriorhodopsin molecules involved in phototransformations.
Об авторах
V. Oleinikov
National Research Nuclear University MEPhI (Moscow Engineering Physics Institute); Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Автор, ответственный за переписку.
Email: voleinik@mail.ru
Россия, Moscow, 115409; Moscow, 117997
E. Lukashev
Moscow State University
Email: voleinik@mail.ru
Россия, Moscow, 119992
S. Zaitsev
Skryabin State Academy of Veterinary Medicine and Biotechnology
Email: voleinik@mail.ru
Россия, Moscow, 109472
A. Chistyakov
National Research Nuclear University MEPhI (Moscow Engineering Physics Institute); Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: voleinik@mail.ru
Россия, Moscow, 115409; Moscow, 117997
D. Solovyeva
National Research Nuclear University MEPhI (Moscow Engineering Physics Institute); Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: voleinik@mail.ru
Россия, Moscow, 115409; Moscow, 117997
K. Mochalov
National Research Nuclear University MEPhI (Moscow Engineering Physics Institute); Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: voleinik@mail.ru
Россия, Moscow, 115409; Moscow, 117997
I. Nabiev
National Research Nuclear University MEPhI (Moscow Engineering Physics Institute); University of Reims Champagne-Ardenne
Email: voleinik@mail.ru
Россия, Moscow, 115409; Reims, 51100
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