Effect of Bacillus subtilis in feed probiotic on the digestion of fish cultured in cages

Abstract

We studied the activity of the digestive enzymes, qualitative composition, and bacteria genera ratio in the intestinal chyme of carp Cyprinus carpio and sterlet Acipenser ruthenus grown on an artificial diet in cage culture. It was shown that the inclusion of live bacteria Bacillus subtilis in the probiotic ProStor had a positive impact on the size and the structure of the microbial community in intestinal chyme, on the activity of some digestive enzymes, and on the digestibility and the weight gain of carp and sterlet. The total amount of micro-organisms in the carp chyme was by 13% higher and in sterlet it was 3.5 times higher in the experimental groups compared to the control. In the studied species of fish, the increase in the activity of digestive enzymes was accompanied by the decrease in nitrogen, ammonia, and urea. Presumably, it happened because of better absorption of protein and more intensive use of nitrogen metabolites by intestinal microflora for the synthesis of their own biomass. The average daily weight gain of carp and sterlet was 25 and 35% higher, respectively, in the experimental groups than in the control.