Email this article Analysis of an association of TNF –308G>A polymorphism with a risk for pulmonary sarcoidosis in the Russian population of the Republic of Karelia
- Authors: Malysheva IE1, Topchieva LV1, Tikhonovich EL2, Barysheva OY.3
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Affiliations:
- Институт биологии Карельского научного центра РАН
- Республиканская больница им. В.А. Баранова
- Петрозаводский государственный университет Минздрава России
- Issue: Vol 89, No 3 (2017)
- Pages: 61-64
- Section: Editorial
- URL: https://journal-vniispk.ru/0040-3660/article/view/32203
- DOI: https://doi.org/10.17116/terarkh201789361-64
- ID: 32203
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Abstract
Aim. To analyze an association of TNF –308G>A polymorphism with a risk for pulmonary sarcoidosis (PS) in the Russian population of the Republic of Karelia Subjects and methods. 84 patients with persistent PS and 96 donors without clinical manifestations of this disease (a control group) were examined. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was used to identify alleles and genotypes by the marker of TNF –308G>A polymorphism. The level of transcripts of the above gene in the peripheral blood leukocytes of healthy and sick people was determined by real-time PCR. Results. There were no significant differences in the distribution of allelic and genotypic frequencies by the marker of TNF –308G>A polymorphism between the control and PS patient groups. There was a significant increase in the number of TNF gene transcripts in the peripheral blood leukocytes of patients with PS compared to the controls. At the same time, there were no marked differences in mRNA expression levels in the above gene in the carriers of different genotypes by the marker of TNF –308G>A polymorphism in all the examined groups. Conclusion. The marker of TNF –308G>A polymorphism is unassociated with the risk of PS in the Russian population of the Republic of Karelia. No differences in TNF mRNA levels in the carriers of different genotypes by the above marker may suggest that the found elevated level of transcripts in the above gene in patients with diagnosed with PS is due to the development of the body’s inflammatory responses in this disease.
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##article.viewOnOriginalSite##About the authors
I E Malysheva
Институт биологии Карельского научного центра РАНПетрозаводск, Россия
L V Topchieva
Институт биологии Карельского научного центра РАНПетрозаводск, Россия
E L Tikhonovich
Республиканская больница им. В.А. БарановаПетрозаводск, Россия
O Yu Barysheva
Петрозаводский государственный университет Минздрава РоссииПетрозаводск, Россия
References
- .Саркоидоз: Монография. Под ред. А.А. Визеля. Серия монографий Российского респираторного общества. Гл. ред. серии А.Г. Чучалин. М.: Атмосфера, 2010.
- Тихонович Э.Л., Везикова Н.Н., Маркелова О.А., Малышева И.Е. Эпидемиология, особенности клиники, диагностики и лечения саркоидоза в Карелии. Ученые записки Петрозаводского государственного университета. Серия: Естественные и технические науки. 2015;6(151):67-71.
- McDougal K, Fallin M, Moller D, Song Z, Cutler D, Steiner L, Cutting G. Variation in the lymphotoxin-alpha/tumor necrosis factor locus modifies risk of erythema nodosum in sarcoidosis. The Journal of investigative dermatology. 2009;129(8):1921-1926. doi: 10.1038/jid.2008.456
- Iannuzzi M, Rybicki B, Teirstein A. Sarcoidosis. The New England journal of medicine. 2007;357:2153-2165. doi: 10.1056/NEJMra071714
- Agostini C, Semenzato G. Cytokines in sarcoidosis. Seminars in respiratory infections. 1998;13(3): 184-196.
- Goetz F, Planas J, MacKenzie S. Tumor necrosis factors. Developmental and comparative immunology. 2004;28(5):487-497. doi: 10.1016/j.dci.2003.09.008
- Feng Y, Zhou J, Gu C, Ding Y, Wan H, Ni L, Niu W.Assotiation of six well-characterized polymorphisms in TNF-α and TNF-β genes with sarcoidosis: a meta-analysis. PLoS One. 2013;8(11):e80150. doi: 10.1371/journal.pone.0080150.
- Medica I, Kastrin A, Maver A, Petrlin B. Role of genetic polymorphisms in ACE and TNF-alpha gene in sarcoidosis: a meta-analysis. Journal of human genetics. 2007;52(10):836-847. doi: 10.1007/s10038-007-0185-7
- Song G, Kim J, Lee Y. Associations between TNF-α -308 A/G and lymphotoxin-α +252 A/G polymorphisms and susceptibility to sarcoidosis: a meta-analysis. Molecular biology reports. 2014;41(1):259-267. doi: 10.1007/s11033-013-2859-x
- Wilson A, Symons J, McDowell T, McDevitt H, Duff G. Effects of a polymorphism in the human tumor necrosis factor alpha promoter on transcriptional activation. Proceedings of the National Academy of Sciences of the United States of America. 1997;94(7):3195-3199.
- Seitzer U, Swider C, Stüber F, Suchnicki K, Lange A, Richter E, Zabel P, Müller-Quernheim J, Flad HD, Gerdes J Tumour necrosis factor alpha promoter gene polymorphism in sarcoidosis. Cytokine. 1997;9:787-790. doi: 10.1006/cyto.1997.0224
- Топчиева Л.В., Малышева И.Е., Курбатова И.В., Корнева В.А., Степанова А.И., Немова Н.Н. Анализ ассоциации полиморфного маркера — 308G>A гена TNF с развитием эссенциальной артериальной гипертензии у жителей Карелии. Медицинская генетика. 2014;13(12):11-15.
- Helmig S, Aliahmadi N, Stephan P, Dцhrel J, Schneider J. TNF-α -308 genotypes are associated with TNF-α and TGF-β? mRNA expression in blood leucocytes of humans. Cytokine. 2011;53(3):306-310. doi: 10.1016/j.cyto.2010.11.018
- Sharma S, Ghosh B, Sharma S K. Assotiation of TNF polymorphisms with sarcoidosis, its prognosis and tumour necrosis factor (TNF)-α levels in Asian Indians. Clinical and experimental immunology. 2008;151(2):251-259. doi: 10.1111/j.1365-2249.2007.03564.x
- Ito M, Takahashi H, Fuse K, Hirono S, Washizuka T, Kato K, Yamazaki F, Inano K, Furukawa T, Komada M, Aizawa Y. Polymorphisms of tumor necrosis factor-alpha and interleukin-10 genes in Japanese patients with idiopathic dilated cardiomyopathy. Japanese heart journal. 2000;41(2):183-191. doi: 10.1536/jhj.41.183
- Pinto J, Dias V, Zoller H, Porto G, Carmo H, Carvalho F, de Sousa M. Hepcidin messenger RNA expression in human lymphocytes. Immunology. 2010;130(2):217-230. doi: 10.1111/j.1365-2567.2009.03226.x
- Swider C, Schnittger L, Bogunia-Kubik K, Gerdes J, Flad H, Lange A, Seitzer U. TNF-alpha and HLA-DR genotyping as potential prognostic markers in pulmonary sarcoidosis. Europeancytokine network. 1999;10(2):143-146. doi: 10.1006/cyto.1997.0224
- Mrazek F., Holla L, Huturova B, Znojil V,Vasku A, Kolek V, Welsh K, Vacha J, du Bois R, Petrek M. Association of tumour necrosis factor-alpha, lymphotoxin-alpha and HLA-DRB1 gene polymorphisms with Löfgren’s syndrome in Czech patients with sarcoidosis. Tissue Antigens. 2005;65(2):163-171. doi: 10.1111/j.1399-0039.2005.00370.x
- Li Z, Chen W, Hou X, Yu R. Detection of TNF-alpha and NCF in the serum and BALF of patients with sarcoidosis and evaluate their clinical significance. Zhonghua jie he he hu xi za zhi. 199922(1):37-39.
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