Email this article Prevalence ofmycelial fungi in a hematological hospital
- Authors: Petrova NA1, Klyasova GA1, Funygina LP1
-
Affiliations:
- ГНЦ РАМН, Москва
- Issue: Vol 78, No 7 (2003)
- Pages: 58-63
- Section: Editorial
- URL: https://journal-vniispk.ru/0040-3660/article/view/33371
- ID: 33371
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Abstract
Aim. To study prevalence, quantity and species of mycelial fungi in the air of a hematological hospital.
Material and methods. Air samples in the hematological departments were taken monthly by PU- IB
device in the volume 250 l/min. Seeding and identification of mycelial fungi were made on the
Chapeck medium.
Results. Tests for mycelial fungi in the air was 95%. Dominating species were the following: Penicillium
spp., Cladosporium spp., Aspergillus spp. The fungal contamination was seasonal. Maximal isolation
of Penicillium spp was seen in winter and autumn. Aspergillus spp. is represented with 13 species
of which most frequent were A.versicolor, A. niger, A. fumigatus, A. ochraceus, A. flavus. Pathological
material from 19 patients contained Aspergillus spp.: A. fumigatus, A. flavus, A. niger. The isolation
peak was in autumn-winter and coincided with maximal isolation of fungal spores from the air. The
analysis of air samples taken in the wards where patients had Aspergillus spp. In biomaterials showed
that concentration of spores Aspergillus spp. was higher than in nearby wards and corridor: 45.6 CPU/M3 vs 18.8
CFU/m3 and 24.7 CFU/M3, respectively. However, morphologically identical strains (patient-air)
were recognized only in 4 cases from the nearby wards and corridor. None of the air samples
taken in the ward of the patient contained identical species. Minimal amount of the spores of micromycetes
was registered in the wards furnished with ventilation with laminar airflow. Most contaminated
was the air of 4 bed wards in the old building.
Conclusion. Mycological monitoring of the air in hematological departments determined the structure
of mycelial fungi complexes. Though ambient air in the wards is full of fungi, detection of morphologically
identical strains causing invasive mycosis in immunocompromised patients is rare. Further studies
are necessary.
Material and methods. Air samples in the hematological departments were taken monthly by PU- IB
device in the volume 250 l/min. Seeding and identification of mycelial fungi were made on the
Chapeck medium.
Results. Tests for mycelial fungi in the air was 95%. Dominating species were the following: Penicillium
spp., Cladosporium spp., Aspergillus spp. The fungal contamination was seasonal. Maximal isolation
of Penicillium spp was seen in winter and autumn. Aspergillus spp. is represented with 13 species
of which most frequent were A.versicolor, A. niger, A. fumigatus, A. ochraceus, A. flavus. Pathological
material from 19 patients contained Aspergillus spp.: A. fumigatus, A. flavus, A. niger. The isolation
peak was in autumn-winter and coincided with maximal isolation of fungal spores from the air. The
analysis of air samples taken in the wards where patients had Aspergillus spp. In biomaterials showed
that concentration of spores Aspergillus spp. was higher than in nearby wards and corridor: 45.6 CPU/M3 vs 18.8
CFU/m3 and 24.7 CFU/M3, respectively. However, morphologically identical strains (patient-air)
were recognized only in 4 cases from the nearby wards and corridor. None of the air samples
taken in the ward of the patient contained identical species. Minimal amount of the spores of micromycetes
was registered in the wards furnished with ventilation with laminar airflow. Most contaminated
was the air of 4 bed wards in the old building.
Conclusion. Mycological monitoring of the air in hematological departments determined the structure
of mycelial fungi complexes. Though ambient air in the wards is full of fungi, detection of morphologically
identical strains causing invasive mycosis in immunocompromised patients is rare. Further studies
are necessary.
About the authors
N A Petrova
ГНЦ РАМН, МоскваГНЦ РАМН, Москва
G A Klyasova
ГНЦ РАМН, МоскваГНЦ РАМН, Москва
L P Funygina
ГНЦ РАМН, МоскваГНЦ РАМН, Москва
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