The Cytotoxic Effects of Thymol as the Major Component of Trachyspermum ammi on Breast Cancer (MCF-7) Cells
- Authors: Seresht H.R.1, Albadry B.J.2, Al-mosawi A.K.3, Gholami O.3, Cheshomi H.3,4
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Affiliations:
- Traditional and Complementary Medicine Research Center, Sabzevar University of Medical Sciences
- Department of Biology, Science of College, University of Thi Qar
- Cellular and Molecular Research Center, Sabzevar University of Medical Sciences
- Department of Biology, Faculty of Science, Ferdowsi University of Mashhad
- Issue: Vol 53, No 2 (2019)
- Pages: 101-107
- Section: Article
- URL: https://journal-vniispk.ru/0091-150X/article/view/245622
- DOI: https://doi.org/10.1007/s11094-019-01961-w
- ID: 245622
Cite item
Abstract
Natural phenolic compounds have inhibition effects on various malignancies. Thymol is one of these compounds present in several plant sources such as ajowan (Trachyspermum ammi) fruits. In this study, thymol was evaluated for its potential cytotoxic activity as well as its effect on apoptotic gene expression in breast cancer cell line. We used the GC-MS technique to identify the essential oil constituents of ajowan. MCF-7 cells were treated by various concentrations of thymol and half maximum inhibitory concentration (IC50) was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. In addition, morphological alterations and changes of P21, P53 and Mcl-1 gene expression in MCF-7 cells were investigated by microscopic examination and real-time RT-PCR assay. Data from MTT assay indicated that the IC50 values of thymol on MCF-7 cells for 48 h and 72 h were 54 and 62 μg/mL, respectively. Furthermore, this compound significantly affected gene expressions of P53 and P21, but not Mcl-1. Thymol can induce the apoptosis process in MCF-7, and hence it can be considered an anticancer agent in the future.
Keywords
About the authors
Hasan Rezaei Seresht
Traditional and Complementary Medicine Research Center, Sabzevar University of Medical Sciences
Email: hamid.ch65@gmail.com
Iran, Islamic Republic of, Sabzevar
Bushra Jabbar Albadry
Department of Biology, Science of College, University of Thi Qar
Email: hamid.ch65@gmail.com
Iraq, Thi Qar
Aseel Kamil Mohammed Al-mosawi
Cellular and Molecular Research Center, Sabzevar University of Medical Sciences
Email: hamid.ch65@gmail.com
Iran, Islamic Republic of, Sabzevar
Omid Gholami
Cellular and Molecular Research Center, Sabzevar University of Medical Sciences
Email: hamid.ch65@gmail.com
Iran, Islamic Republic of, Sabzevar
Hamid Cheshomi
Cellular and Molecular Research Center, Sabzevar University of Medical Sciences; Department of Biology, Faculty of Science, Ferdowsi University of Mashhad
Author for correspondence.
Email: hamid.ch65@gmail.com
Iran, Islamic Republic of, Sabzevar; Mashhad
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