Polyphasic taxonomic analysis of Bacillus sp. strain C6—the antagonist of phytopathogenic microorganisms

Abstract

Polyphasic taxonomic analysis was carried out for Bacillus sp. strain C6, as the antagonist of phytopathogenic bacteria and micromycetes. The combination of cultural, morphological, physiological, and biochemical properties of the strain has enabled researchers to refer it to the Bacillus subtilis group. It has been shown that the fatty acids of the strain’s cell walls were predominantly represented by branched iso- and anteiso-C15:0 and C17:0 fatty acids (over 85%), which was typical for the Bacillus amyloliquefaciens species. The molecular genetic analysis carried out on the nucleotide sequence of the 16S rRNA gene, and the profiling of polymorphic nucleotides have enabled researchers to refer the strain in question to Bacillus amyloliquefaciens subsp. plantarum.