Chromosomal DNA balance in human stem cell line 4BL

Abstract

Ploidy of a chromosome set and some regular structural aberrations in the new human 4BL cell line by passage 205 have been characterized in the previous cytogenetic studies. The purpose of this study was to investigate, using the array CGH and FISH methods, the nature of regular monosomies in particular homologous pairs. Structural aberrations were detected in all the chromosome pairs distinguished as monosomies according to classical cytogenetic analyses. The most notable alterations have been detected in chromosomes 2, 4, 10, 13, and 17. Massive genetic material losses were a probable cause for the monosomy of chromosomes 4, 10, 13, and 17. The monosomy of the second pair of chromosomes was caused by a substantial transformation in one of the homologs typified as multiple duplications and the formation of a derivative—der(2)t(2;?)(q21;?). The application of array CGH aided us in identifying the regions of structural aberrations in chromosomes 2, 4, 10, 13, and 17, that allowed a more accurate identification with the use of the multicolor FISH method. The obtained results confirm the hypothesis concerning a coordinated emergence of deletions and duplications and their stabilizing effect on transformed chromosomes.