Morphogenesis in vitro in maize inbred lines from the Lancaster heterotic group

Abstract

The genotypic specificities of morphogenesis and regeneration have been studied in vitro in five maize inbred lines from the breeding-promising Lancaster heterotic group, compared with the representatives of other heterotic groups, that is, PLS61, A188, and Chi31. It has been shown that the ratio between the types of morphogenesis, such as organogenesis and embryoidogenesis, in a callus culture is predetermined by the explant genotype and sucrose concentrations in the medium for callusogenesis. The frequency of embryoidogenesis as the most efficient type of morphogenesis, considering further regeneration, was, on average, 40.0 ± 12.8% for maize inbreds from the Lancaster heterotic group and only 14.0 ± 4.0% from other heterotic groups. A sucrose concentration at a level of 30 g/L in the medium for callusogenesis further supported the process of regeneration through embryoidogenesis in inbreds of the Lancaster heterotic group at a level of 26.5 ± 15.4%, while the sucrose concentration of 60 g/L promoted regeneration up to a level of 57.7 ± 19.8%. Contents of sucrose in the medium for callusogenesis in the inbreds representing other heterotic groups did not affect the process of regeneration, and the level of embryoidogenesis with sucrose at the concentrations of 30 and 60 g/L was, respectively, 11.0 ± 7.0 and 15.0 ± 4.8%.