SSR Marker Association with Variation of Stomatal Guard Cell Length in Bread Wheat

Abstract

The aim of the present study was to identify associations between allelic variants of microsatellite markers (SSRs) and genetic polymorphism with regard to “stomatal guard cell length” (SGCL) trait in a population of recombinant-inbred lines (RILs) of bread winter wheat. Cytological analysis of SGCL in leaf epidermis, polymerase chain reaction, and statistical calculations were performed in accordance with conventional procedures. Genetic diversity related to the SGCL trait was detected in an F₇ Luzanivka odes’ka/Odes’ka chervonokolosa wheat RIL population. A significant effect of allelic differences in SSR loci in the four triple combinations of microsatellite loci on SGCL variation was demonstrated.