Email this article The Effect of Platelet-Rich Plasma on Morphogenesis and Expression of Chondrogenic Marker Genes by Chondrocyte-Like Cells of the Rat Nucleus Pulposus in Vitro
- Authors: Pedachenko E.G.1, Vasilyeva I.G.1, Khizniak M.V.1, Chopyck N.G.1, Oleksenko N.P.1, Shuba I.N.1, Tsjubko O.I.1, Galanta O.S.1, Snitsar N.D.1, Makarova T.A.1, Dmytrenko A.B.1
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Affiliations:
- Romodanov Institute of Neurosurgery, Academy of Medical Sciences of Ukraine
- Issue: Vol 53, No 2 (2019)
- Pages: 124-131
- Section: Article
- URL: https://journal-vniispk.ru/0095-4527/article/view/174052
- DOI: https://doi.org/10.3103/S0095452719020087
- ID: 174052
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Abstract
The aim of the study was to investigate the effect of 3, 10, and 20% of platelet-rich rat plasma (PRP) on the morphological characteristics of chondrocyte-like cells of the rat nucleus pulposus (NP) and the expression of chondrogenic genes ACAN, COLII, GPC3, MGP, PTN, ANXA3, and VIM in vitro. A dose-dependent increase of NP cell viability was observed in cultures supplemented with 3, 10, and 20% PRP. Platelets showed tropicity to blast-like cells in culture and formed adhesion zones. Differentiation of blast-like cells was observed and dividing cells were identified on day 14 of cultivation with 20% PRP. PRP prevented the decrease of chondrogenic gene expression in a dose-dependent manner, with 20% PRP being the most efficient. Expression of the majority of chondrogenic genes studied was observed in these cultures: the expression of COLII increased 2.9 times (P = 0.002); that of VIM, 2.1 times (P = 0.0003); that of ANXA3, 2.3 times (P = 0.0003); that of GPC3, 1.4 times (P = 0.0011); that of PTN, 1.6 times (P = 0.0017); and that of ACAN, 1.2 times (P = 0.0606).
About the authors
E. G. Pedachenko
Romodanov Institute of Neurosurgery, Academy of Medical Sciences of Ukraine
Author for correspondence.
Email: pedach@i.ua
Ukraine, Kyiv, 04050
I. G. Vasilyeva
Romodanov Institute of Neurosurgery, Academy of Medical Sciences of Ukraine
Author for correspondence.
Email: vigvasileva@gmail.com
Ukraine, Kyiv, 04050
M. V. Khizniak
Romodanov Institute of Neurosurgery, Academy of Medical Sciences of Ukraine
Author for correspondence.
Email: Khyzhnyak63@gmail.com
Ukraine, Kyiv, 04050
N. G. Chopyck
Romodanov Institute of Neurosurgery, Academy of Medical Sciences of Ukraine
Author for correspondence.
Email: natalia.chopyck@gmail.com
Ukraine, Kyiv, 04050
N. P. Oleksenko
Romodanov Institute of Neurosurgery, Academy of Medical Sciences of Ukraine
Author for correspondence.
Email: n.oleksenko@gmail.com
Ukraine, Kyiv, 04050
I. N. Shuba
Romodanov Institute of Neurosurgery, Academy of Medical Sciences of Ukraine
Author for correspondence.
Email: irina_shuba@ukr.net
Ukraine, Kyiv, 04050
O. I. Tsjubko
Romodanov Institute of Neurosurgery, Academy of Medical Sciences of Ukraine
Author for correspondence.
Email: tsjubkoolga@gmail.com
Ukraine, Kyiv, 04050
O. S. Galanta
Romodanov Institute of Neurosurgery, Academy of Medical Sciences of Ukraine
Author for correspondence.
Email: lenagalanta@gmail.com
Ukraine, Kyiv, 04050
N. D. Snitsar
Romodanov Institute of Neurosurgery, Academy of Medical Sciences of Ukraine
Author for correspondence.
Email: 1866600@online.ua
Ukraine, Kyiv, 04050
T. A. Makarova
Romodanov Institute of Neurosurgery, Academy of Medical Sciences of Ukraine
Author for correspondence.
Email: tatyana.makarova77799@gmail.com
Ukraine, Kyiv, 04050
A. B. Dmytrenko
Romodanov Institute of Neurosurgery, Academy of Medical Sciences of Ukraine
Author for correspondence.
Email: angelikdmiz.bor@gmail.com
Ukraine, Kyiv, 04050
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