Biochip with Cells of Brush Polymers Carrying Carboxyl Groups for DNA Analysis

R. A. Miftakhov; Мифтахов Р. А.; T. V. Nasedkina; Наседкина Т. В.; A. S. Zasedatelev; Заседателев А. С.; V. E. Shershov; Шершов В. Е.; V. E. Kuznetsova; Кузнецова В. Е.; A. I. Levashova; Левашова А. И.; S. A. Lapa; Лапа С. А.; V. A. Vasiliskov; Василисков В. А.; A. Yu. Ikonnikova; Иконникова А. Ю.; A. V. Chudinov; Чудинов А. В.

doi:10.31857/S0132342323050044

Biochip with Cells of Brush Polymers Carrying Carboxyl Groups for DNA Analysis

作者: Miftakhov R.A.¹, Nasedkina T.V.¹, Zasedatelev A.S.¹, Shershov V.E.¹, Kuznetsova V.E.¹, Levashova A.I.¹, Lapa S.A.¹, Vasiliskov V.A.¹, Ikonnikova A.Y.¹, Chudinov A.V.¹
隶属关系:
1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
期: 卷 49, 编号 6 (2023)
页面: 641-648
栏目: ПИСЬМА РЕДАКТОРУ
URL: https://journal-vniispk.ru/0132-3423/article/view/148163
DOI: https://doi.org/10.31857/S0132342323050044
EDN: https://elibrary.ru/ARRVXQ
ID: 148163

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详细

A method for manufacturing biochips by photolithography with hydrogel cells made of brush copolymers based on acrylic acid and acrylamide, fixed at one end on the surface of a polymer substrate has been developed. Hydrogel cells with reactive carboxyl groups were used for covalent immobilization of oligonucleotide probes. The effectiveness of the method was demonstrated in the hybridization analysis of DNA by targets of different lengths of the sequence site 7 of the exon of the human ABO gene.

关键词

biochips, brush polymers, immobilization of oligonucleotides, DNA hybridization analysis

参考

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