A Short-Term Method for Assessing the Genotoxicity of Soil As a Solid-Phase Body Based on the Allium Test

Abstract

A design of a short-term experiment for determining the indices of the cyto- and genotoxicity of soils with hydrophobic organic pollutants has been developed using the Allium test and soil-plate method. A procedure of soil sample preparation, biotester material pretreatment, and the 120-h solid-phase phytotest procedure itself, as well as instrumentation for calculating the results of cytogenetic analysis, have been proposed. The technique has been tested for assessing the cyto(geno)toxicity of urbanozem containing persistent organic pollutants, as well as for the toxicometry of brown forest soil of oil production area.