Distribution of progesterone receptors and the membrane component of progesterone receptor in various organs and tissues of male and female rats

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Abstract

Progesterone regulates reproductive processes and affects many functions of various non-reproductive organs. Its effects in mammals and humans are mediated by nuclear (nPRs) and membrane progesterone receptors (mPRs). The action of progesterone through different types of receptors may differ significantly and has tissue specific features. The expression of known types and subtypes of progesterone receptors in the tissues of male and female rats has been studied fragmentarily. The purpose of our work was to study the expression of five mPRs genes, as well as the nPRs gene and the membrane component of the progesterone receptor PGRMC I in the reproductive organs and in 17 non-reproductive tissues of male and female rats using reverse transcription followed by real-time PCR. In this study, it was shown that a high level of nPRs gene expression in rats is found not only in reproductive organs of females (uterus, ovary, mammary glands), but also in seminal vesicles of males, in the brain and trachea of both sexes, in blood vessels, and in the pancreas of females. The highest level of expression of mPRs genes of all subtypes was found in the testes, while expression of the gene encoding nPRs was practically undetectable in them. Expression of genes encoding mPRs was also detected in the liver and spleen of male and female rats, while expression of the gene encoding nPRs was at background levels. Virtually no expression of nPRs, mPRs, and membrane component of progesterone receptor (PGRMC I) genes was detected in muscle, and its level was very low in the heart in animals of both sexes. We found sex-specific differentiation of nuclear and membrane receptor mRNA levels in rats in non-reproductive tissues, characterized by a predominance of nPRs transcripts and three subtypes of mPRs (α, β, δ) in females and two subtypes of mPRs (γ, ε) in males. Data on the presence of progesterone receptors in tissues not involved in reproduction confirm the effect of progesterone on these organs. High levels of mRNA for various progesterone receptors in the tissues of male rats, such as the pancreas, lungs, kidney, and trachea, indicate an important physiological role of progestins not only in females, but also in males, which is still poorly understood. The work also discusses the known functions of progesterone receptors in the tissues studied.

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About the authors

A. D. Dmitrieva

Lomonosov Moscow State University

Email: schelkunova-t@mail.ru

Biological Department

Russian Federation, Moscow, 119991

I. A. Morozov

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: schelkunova-t@mail.ru
Russian Federation, Moscow, 119991

A. M. Karhov

Lomonosov Moscow State University

Email: schelkunova-t@mail.ru

Biological Department

Russian Federation, Moscow, 119991

P. M. Rubtsov

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: schelkunova-t@mail.ru
Russian Federation, Moscow, 119991

O. V. Smirnova

Lomonosov Moscow State University

Email: schelkunova-t@mail.ru

Biological Department

Russian Federation, Moscow, 119991

T. A. Shchelkunova

Lomonosov Moscow State University

Author for correspondence.
Email: schelkunova-t@mail.ru

Biological Department

Russian Federation, Moscow, 119991

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2. Fig. 1. Expression level of nPRs mRNA in organs of male and female Wistar rats (non-reproductive tissues, n = 3, reproductive n = 5). Data are presented as mean ± standard deviation.

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3. Fig. 2. Expression level of mPRα mRNA in organs of male and female Wistar rats (non-reproductive tissues, n = 3, reproductive n = 5). Data are presented as mean ± standard deviation.

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4. Fig. 3. Expression level of mPRβ mRNA in organs of male and female Wistar rats (non-reproductive tissues, n = 3, reproductive n = 5). Data are presented as mean ± standard deviation.

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5. Fig. 4. Expression level of mPRγ mRNA in organs of male and female Wistar rats (non-reproductive tissues, n = 3, reproductive n = 5). Data are presented as mean ± standard deviation.

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6. Fig. 5. Expression level of mPRδ mRNA in organs of male and female Wistar rats (non-reproductive tissues, n = 3, reproductive n = 5). Data are presented as mean ± standard deviation.

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7. Fig. 6. Expression level of mPRε mRNA in organs of male and female Wistar rats (non-reproductive tissues, n = 3, reproductive n = 5). Data are presented as mean ± standard deviation.

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8. Fig. 7. PGRMC I mRNA expression levels in organs of male and female Wistar rats (non-reproductive tissues, n = 3, reproductive n = 5). Data are presented as mean ± standard deviation.

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9. Fig. 8. Expression levels of mPRs and PGRMC I mRNA in the liver and spleen of female and male Wistar rats (n = 3) and in the testes (n = 5). Data are presented as mean ± standard deviation.

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