Preliminary Exposure to Histone Deacetylase Inhibitors Changes the Direction of Human IPSCs Differentiation with the Formation of Cardiospheres Instead of Skin Organoids

V. K. Abdyev; Абдыев В. К.; A. A. Riabinin; Рябинин А. А.; E. D. Erofeeva; Ерофеева Е. Д.; M. D. Pankratova; Панкратова М. Д.; E. A. Vorotelak; Воротеляк Е. А.; A. V. Vasiliev; Васильев А. В.

doi:10.31857/S0475145023060022

Preliminary Exposure to Histone Deacetylase Inhibitors Changes the Direction of Human IPSCs Differentiation with the Formation of Cardiospheres Instead of Skin Organoids

Authors: Abdyev V.K.¹, Riabinin A.A.¹, Erofeeva E.D.², Pankratova M.D.¹^,2, Vorotelak E.A.¹, Vasiliev A.V.¹^,2
Affiliations:
1. Koltsov Institute of Developmental Biology of Russian Academy of Sciences
2. Lomonosov Moscow State University, Faculty of Biology
Issue: Vol 54, No 6 (2023)
Pages: 389-396
Section: Short communications
URL: https://journal-vniispk.ru/0475-1450/article/view/232035
DOI: https://doi.org/10.31857/S0475145023060022
EDN: https://elibrary.ru/HKRZYI
ID: 232035

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Abstract
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Abstract

Pluripotent stem cells (PSCs) are a unique cell type that can differentiate into all cell types in the body. In PSC culture, subpopulations with different levels of pluripotency may exist, which leads to different results during their differentiation. One of the key factors that determine the state of pluripotency and influence the differentiation potential of PSCs is the epigenetic state of cells, including the level of histone deacetylation. Activation of histone deacetylase (HDAC) in human and mouse PSCs increases the percentage of heterochromatin. In this work, we used a protocol for the differentiation of embryoid bodies from induced human pluripotent hIPSC cells, designed for the formation of ectoderm and neuroectoderm with their subsequent development into skin organoids. However, after hIPSCs were exposed to HDAC inhibitors (sodium butyrate and valproic acid), the direction of their differentiation changed: mesoderm was formed, which subsequently developed into contracting cardiospheres.

Keywords

hIPSCs, directed differentiation, HDAC, skin organoid, ectoderm, neuroectoderm, HDACi, cardiospheres

References

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