MORPHOLOGICAL ANALYSIS OF HEPATITIS B VIRUS WITH ESCAPE MUTATIONS IN S-gene G145R AND S143L
- 作者: Konopleva M.V.1, Sokolova M.V.1, Shevlyagina N.V.1, Bazhenov A.I.2, Fel’Dsherova A.A.1, Krymskij M.A.3, Borisova V.N.3, Semenenko T.A.1, Nesterenko V.G.1, Suslov A.P.1
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隶属关系:
- Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya
- N.V. Sklifosovsky Research Institute for Emergency Medicine of Moscow Healthcare Department
- ZAO NPK Combiotech
- 期: 卷 62, 编号 3 (2017)
- 页面: 119-128
- 栏目: ORIGINAL RESEARCH
- URL: https://journal-vniispk.ru/0507-4088/article/view/117932
- DOI: https://doi.org/10.18821/0507-4088-2017-62-3-119-128
- ID: 117932
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Background. In terms of serological properties and immunization, the wild type of HBsAg HBV and its G145R mutant behave as different antigens. This testifies to serious structural changes, which presumably could have a significant impact on the morphogenesis of virions and subviral particles. Nevertheless, morphological and ultrastructural investigations of HBV with G145R mutation have not been carried yet. Objectives. Research of structural and morphological organization of HBV in the presence of the G145R escape mutation. Methods. Studies of sera, purified viruses and recombinant HBsAg were carried out by transmission electron microscopy by the method of negative staining and indirect reaction of immunelabeling using monoclonal antibodies of different specificity. Specimens of wild type HBV and HBV with S143L mutation obtained in an identical manner were used as the control. Results. The presence of typical virus particles of HBV was shown in the specimens of wild strain and HBV with S143L mutation. Specimens of HBV with G145R mutation were characterized by expressed morphological heterogeneity. In the initial serum and in the specimen of purified virus containing G145R mutant, large oval particles 60-70 nm and up to 200 nm in size, respectively, were found. The presence of antigen structures of HBV in all heterogeneous forms was confirmed. It was shown that forming of subviral particles in the process of expression of the recombinant HBsAg with G145R mutation depends on conditions of expression and purification of the protein. They can vary from well-formed circular and oval particles to practically unstructured fine-grained masses. Conclusion. Direct data on the impact of G145R escape-mutation in S-gene, in contrast to S143L mutation, on the morphogenesis of virions and subviral particles of HBV were obtained.
作者简介
M. Konopleva
Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya
编辑信件的主要联系方式.
Email: maria-konopleva@rambler.ru
俄罗斯联邦
M. Sokolova
Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya
Email: noemail@neicon.ru
俄罗斯联邦
N. Shevlyagina
Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya
Email: noemail@neicon.ru
俄罗斯联邦
A. Bazhenov
N.V. Sklifosovsky Research Institute for Emergency Medicine of Moscow Healthcare Department
Email: noemail@neicon.ru
俄罗斯联邦
A. Fel’Dsherova
Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya
Email: noemail@neicon.ru
俄罗斯联邦
M. Krymskij
ZAO NPK Combiotech
Email: noemail@neicon.ru
俄罗斯联邦
V. Borisova
ZAO NPK Combiotech
Email: noemail@neicon.ru
俄罗斯联邦
T. Semenenko
Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya
Email: noemail@neicon.ru
俄罗斯联邦
V. Nesterenko
Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya
Email: noemail@neicon.ru
俄罗斯联邦
A. Suslov
Federal Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya
Email: noemail@neicon.ru
俄罗斯联邦
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