Experience of application of IgY-technology for laboratory diagnostics of viral infections
- 作者: Ivanov A.P.1, Klebleeva T.D.1, Ivanova O.E.1,2
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隶属关系:
- Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences
- I.M. Sechenov First Moscow State Medical University
- 期: 卷 65, 编号 1 (2020)
- 页面: 21-26
- 栏目: ORIGINAL RESEARCH
- URL: https://journal-vniispk.ru/0507-4088/article/view/118021
- DOI: https://doi.org/10.36233/0507-4088-2020-65-1-21-26
- ID: 118021
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详细
Introduction. The well-known advantages of class Y antibodies (IgY) from egg yolks of immunized hens in comparison with class G antibodies (IgG) of laboratory animals traditionally used in laboratory diagnosis of infectious diseases determine the stable interest of researchers in using IgY for these purposes (IgY technology) . Over the past 20 years, the obvious benefits of IgY technology have been demonstrated for a number of viral and bacterial infections. Goals and objectives. Construction of ELISA systems based on specific IgY for laboratory diagnosis of infections caused by tick-borne encephalitis virus, yellow fever virus, poliovirus.
Material and methods. Obtaining yolk preparations of immunized chickens, obtaining highly purified IgY preparations (salting out, affinity chromatography), constructing ELISA systems for determining virus-specific antigens, testing the parameters of ELISA systems.
Results and discussion. For the first time in laboratory practice, ELISA systems based on the use of specific polyclonal IgY were designed for laboratory diagnosis of topical human viral infections caused by flaviviruses and enteroviruses: determination of antigens of tick-borne encephalitis virus, yellow fever virus, 3 types of poliovirus. It was experimentally shown that these ELISA systems have high sensitivity and specificity, which allows them to be used for the semiquantitative and quantitative determination of antigens of these viruses in various materials (infected cell cultures, vaccines, etc.).
Conclusion. The ELISA systems developed on the basis of specific IgY for determination of viral antigens can be effectively used for laboratory diagnosis of a number of viral infections, for the validation and control of vaccine preparations.
作者简介
A. Ivanov
Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences
编辑信件的主要联系方式.
Email: ivanovalexander1@gmail.com
ORCID iD: 0000-0003-4576-7136
Alexander P. Ivanov, Dr. Sci. Med., 1st category technologist, Department for the development and implementation of innovative technologies.
Moscow, 108819
俄罗斯联邦T. Klebleeva
Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences
Email: fake@neicon.ru
ORCID iD: 0000-0003-4288-6818
Moscow, 108819 俄罗斯联邦
O. Ivanova
Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences; I.M. Sechenov First Moscow State Medical University
Email: fake@neicon.ru
ORCID iD: 0000-0003-1784-4827
108819, Russia
119991, Russia 俄罗斯联邦
参考
- Schade R., Calzado E.G., Sarmiento R., Chacana P.A., Porankiewicz-Asplund J., Terzolo H.P. Chicken egg yolk antibodies (IgYtechnology): a review of progress in production and use in research and human and veterinary medicine. Altern. Lab. Anim. 2005; 33(2): 129-54. http://doi.org/10.1177/026119290503300208
- Schade R., Hendriksen C., Staak C., Erhard M. The production of avian (egg yolk) antibodies: IgY. The report and recommendations of ECVAM workshop 21. Altern. Lab. Anim. 1997; 24(6): 925-34.
- Haak-Frendscho M. Why IgY? Chicken polyclonal antibody, an appealing alternative. Promega Notes Magazine. 1994; 46: 11-4.
- Иванов А.П., Варгин В.В. Препараты IgY из куриного желтка как иммунные реагенты к вирусу клещевого энцефалита. Вопросы вирусологии. 2010; 55(2): 46-8.
- Akita E.M., Nakai S. Comparison of four purification methods for the production of immunoglobulins from eggs laid by hens immunized with an enterotoxigenic E. coli strain. J. Immunol. Meth. 1993; 160(2): 207-14. http://doi.org/10.1016/0022-1759(93)90179-b
- World Health Organization. Manual for the virological investigation of polio [WHO/EPI/GEN97.01]. Geneva: WHO; 1997.
- Mercier-Delarue S., Durier C., Colin de Verdière N., Poveda JD., Meiffrédy V., Garcia MDF., et al. Screening test for neutralizing antibodies against yellow fever virus, based on flavivirus pseudotype. PLoS One. 2017; 12(5): e0177882. http://doi.org/10.1371/journal.pone.0177882
- Harlow E., Lane D. Antibodies: a Laboratory Manual. New York: Cold Spring Harbor Laboratory Press; 1988.
- Иванов А.П., Козлов В.Г., Клеблеева Т.Д., Иванова О.Е., Киктенко А.В. Система иммуноферментного анализа на основе специфических антител класса Y (IgY) из яичных желтков для количественного определения D-антигена в инактивированных полиовирусных вакцинах. Вопросы вирусологии. 2014; 59(6): 39-42.
- Ivanov A.P., Bashkirtsev V.N., Tkachenko E.A. Enzyme-linked immunosorbent assay for detection of arenaviruses. Arch. Virol. 1981; 67(1): 71-4. http://doi.org/10.1007/BF01314603
- Иванов А.П., Клеблеева Т.Д., Иванова О.Е., Ипатова Е.Г., Гмыль Л.В., Ишмухаметов А.А. Экспериментальные подходы к разработке инактивированной полиовирусной вакцины на основе штаммов Сэбина. Эпидемиология и вакцинопрофилактика. 2016; 15(4): 59-64. http://doi.org/10.31631/2073-3046-2016-15-4-59-64
- Иванов А.П., Клеблеева Т.Д., Синюгина А.А., Ишмухаметов А.А. Разработка инактивированной культуральной вакцины против желтой лихорадки. В кн.: Материалы XI Ежегодного Всероссийского Конгресса по инфекционным болезням с международным участием «Инфекционные болезни в современном мире: эволюция, текущие и будущие угрозы». М.; 2019: 68-9.
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