Evaluation of the human sensitivity to smallpox virus by the primary cultures of the monocyte-macrophages

A. S. Zamedyanskaya; Замедянская А. С.; K. A. Titova; Титова К. А.; Al. A. Sergeev; Сергеев Ал. А.; A. S. Kabanov; Кабанов А. С.; L. E. Bulychev; Булычев Л. Е.; Ar. A. Sergeev; Сергеев Ар. А.; D. O. Galakhova; Галахова Д. О.; A. E. Nesterov; Нестеров А. Е.; O. V. Nosareva; Носарева О. В.; L. N. Shishkina; Шишкина Л. Н.; O. S. Taranov; Таранов О. С.; V. V. Omigov; Омигов В. В.; A. P. Agafonov; Агафонов А. П.; A. N. Sergeev; Сергеев А. Н.

doi:10.18821/0507-4088-2016-61-2

Evaluation of the human sensitivity to smallpox virus by the primary cultures of the monocyte-macrophages

Authors: Zamedyanskaya A.S.¹, Titova K.A.¹, Sergeev A.A.¹, Kabanov A.S.¹, Bulychev L.E.¹, Sergeev A.A.¹, Galakhova D.O.¹, Nesterov A.E.¹, Nosareva O.V.¹, Shishkina L.N.¹, Taranov O.S.¹, Omigov V.V.¹, Agafonov A.P.¹, Sergeev A.N.¹
Affiliations:
1. State Research Center for Virology and Biotechnology «Vector»
Issue: Vol 61, No 2 (2016)
Pages: 69-73
Section: ORIGINAL RESEARCH
URL: https://journal-vniispk.ru/0507-4088/article/view/118052
DOI: https://doi.org/10.18821/0507-4088-2016-61-2
ID: 118052

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Abstract

Studies of the primary cultures of granulocytes, mononuclear, and monocyte-macrophage cells derived from human blood were performed using variola virus (VARV) in the doses of 0.001-0.021 PFU/cell (plaques-forming units per cell). Positive dynamics of the virus accumulation was observed only in the monocyte-macrophages with maximum values of virus concentration (5.0-5.5 lg PFU/ml) mainly within six days after the infection. The fact of VARV replication in the monocyte-macrophages was confirmed by the data of electron microscopy. At the same time, virus vaccines when tested in doses 3.3 and 4.2 lg PFU/ml did not show the ability to reproduce in these human cells. The people sensitivity to VARV as assessed from the data obtained on human monocytemacrophages corresponded to ~1 PFU (taking into account the smooth interaction of the virus in the body to the cells of this type), which is consistent to previously found theoretical data on the virus sensitivity. The human susceptibility to VARV assessed experimentally can be used to predict the adequacy of developed smallpox models (in vivo) based on susceptible animals. This is necessary for reliable assessment of the efficiency of development of drugs for treatment and prophylaxis of the smallpox.

Keywords

variola virus, vaccinia virus, human, primary blood cells, 50% infectious dose, virus production

About the authors

A. S. Zamedyanskaya

State Research Center for Virology and Biotechnology «Vector»

Author for correspondence.
Email: zamedyanskaya_as@vector.nsc.ru
ORCID iD: 0000-0002-1177-2693

Alena S. Zamedyanskaya, Junior research scientist

Koltsovo, Novosibirsk region, 630559

Russian Federation

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