Rapid diagnostics of novel coronavirus infection by loop-mediated isothermal amplification

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Abstract

This review presents the basic principles of application of the loop-mediated isothermal amplification (LAMP) reaction for the rapid diagnosis of coronavirus infection caused by SARS-CoV-2. The basic technical details of the method, and the most popular approaches of specific and non-specific detection of amplification products are briefly described. We also discuss the first published works on the use of the method for the detection of the nucleic acid of the SARS-CoV-2 virus, including those being developed in the Russian Federation. For commercially available and published LAMP-based assays, the main analytical characteristics of the tests are listed, which are often comparable to those based on the method of reverse transcription polymerase chain reaction (RT-PCR), and in some cases are even superior. The advantages and limitations of this promising methodology in comparison to other methods of molecular diagnostics, primarily RT-PCR, are discussed, as well as the prospects for the development of technology for the detection of other infectious agents.

About the authors

K. F. Khafizov

FSBI Central Research Institute for Epidemiology of the Federal Service for Surveillance of Consumer Rights Protection and Human Wellbeing (Rospotrebnadzor)

Author for correspondence.
Email: kkhafizov@gmail.com
ORCID iD: 0000-0001-5524-0296

Khafizov Kamil Faridovich - Ph.D. (Biol.), Head of the Scientific Group for the Development of New Diagnostic Methods of the Department for Molecular Diagnostics and Epidemiology.

111123, Moscow, Novogireevskaya str. 3a

Russian Federation

V. V. Petrov

FSBI Central Research Institute for Epidemiology of the Federal Service for Surveillance of Consumer Rights Protection and Human Wellbeing (Rospotrebnadzor)

Email: petrov@pcr.ms
ORCID iD: 0000-0002-3503-2366

Petrov Vadim Viktorovich - Head of the research group for the development of new molecular biological technologies.

111123, Moscow, Novogireevskaya str. 3a

Russian Federation

K. V. Krasovitov

FSBI Central Research Institute for Epidemiology of the Federal Service for Surveillance of Consumer Rights Protection and Human Wellbeing (Rospotrebnadzor)

Email: krasovitov@cmd.su
ORCID iD: 0000-0001-7237-1810

Krasovitov Kirill Vladimirovich - Laboratory research assistant, Research group for the development of new molecular biological technologies.

111123, Moscow, Novogireevskaya str. 3a

Russian Federation

M. V. Zolkina

FSBI Central Research Institute for Epidemiology of the Federal Service for Surveillance of Consumer Rights Protection and Human Wellbeing (Rospotrebnadzor)

Email: mvzolkina@mail.ru
ORCID iD: 0000-0002-2973-9947

Zolkina Maria Vladimirovna - Head of the development.

111123 Moscow, Novogireevskaya str. 3a

Russian Federation

V. G. Akimkin

FSBI Central Research Institute for Epidemiology of the Federal Service for Surveillance of Consumer Rights Protection and Human Wellbeing (Rospotrebnadzor)

Email: vgakimkin@yandex.ru
ORCID iD: 0000-0003-4228-9044

Akimkin Vasily Gennadevich - Academician of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor, Director.

111123, Moscow, Novogireevskaya str. 3a

Russian Federation

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